奥曲肽与胰岛素对人肝癌细胞影响的体外实验研究

Effects of Octreotide and insulin on human hepatoma cell in vitro

目的 旨在探讨胰岛素能否促进人肝癌细胞增殖 ,而奥曲肽对其诱导的增殖活动有无抑制作用。方法 将奥曲肽与胰岛素直接作用于体外培养的人原发性肝癌细胞株 (BEL 740 2 ) ,运用四氮唑盐、直接细胞计数及流式细胞仪测定等方法观察奥曲肽与胰岛素对BEL 740 2细胞株活细胞数及其比值、细胞总数、细胞周期及增殖指数 (PI)的影响。结果 胰岛素 (0～ 5 μg/ml)使BEL 740 2细胞株的细胞总数、活细胞数及其比值增加 ,呈明显的量效关系 ,而奥曲肽 (0～ 2 μg/ml)则使BEL 740 2基础的及胰岛素刺激的细胞总数、活细胞数及其比值降低 ,亦呈明显的量效关系。另外 ,5 μg/ml的胰岛素增加细胞的PI值 ,而 1μg/ml的奥曲肽降低其PI值 (P <0 .0 5 ) ,并对细胞周期同步化的BEL 740 2细胞株产生S期阻滞作用 (P <0 .0 5 )。结论 在体外 ,胰岛素可诱导人肝癌细胞增殖 ,奥曲肽不仅直接抑制肝癌细胞增殖 。

Objective To investigate whether the proliferation of human hepatoma cell can be induced by insulin and which can be inhibited by Octreotide. Methods A human hepatoma cell line BEL7402 was used in this study. Using MTT method, cell count and Flow Cytometry (FCM), the effects of insulin and/or Octreotide on the number of viable cell, its ratio, the total number of cells, cell cycle and proliferative index (PI) in phasesynchronized cells were assayed. Results The cell number, the viable cell number and its ratio in insulin (0～5 μg/ml)treated BEL7402 cells increased, while both in basic and insulininduced ones all decreased when Octreotide 0～2 μg/ml was used, both in dosedependent manner. 1 μg/ml of Octreotide resulted in a reduction of PI which was increased by 5 μg/ml of insulin. Moreover, a S cell cycle block was detectable in phasesynchronised BEL7402 cell treated with 1 μg/ml of Octreotide. Conclusion In vitro, proliferation of human hepatoma cell can be induced by insulin, while Octreotide exerted an inhibitory effects on basic and insulininduced growth of human hepatoma cell.