hsa-miR-206负调控核受体LXRα mRNA的表达水平

hsa-miR-206 Negatively Regulates Nuclear Receptor LXRαmRNA Expression

MicroRNA(miRNA)是在真核生物中发现的一类内源性的具有调控功能的非编码RNA.在动物细胞内,miRNA通常引起目的mRNA的切割或阻碍其翻译过程,从而抑制目的基因的表达.本研究发现,hsa-miR-206通过与LXRα3′UTR直接相互作用,以抑制细胞中LXRα的mRNA水平,下调LXRα的转录活性,并最终使LXRα配体GW3965对LXR的目的基因SCD-1的诱导作用受到抑制.这些结果表明,hsa-miR-206能够负调控LXRα的表达及转录活性.

Liver X receptor （LXR） is a member of the nuclear receptor superfamily of ligand-activated transcription factors.LXRs exhibit regulatory effects on diverse physiological functions,ranging from cholesterol and lipid metabolism to anti-inflammation,hepatobiliary diseases,and steroid hormone biosynthesis and metabolism.miRNAs are endogenous non-coding RNAs found in eukaryotes.In mammalian cells,miRNAs usually inhibit target genes expression by splicing their target messenger RNA or blocking mRNA translations.In our present study,we found hsa-miR-206 could interact with LXRα 3＇ untranslated region through direct binding with its response element.hsa-miR-206 inhibited cellular LXRα mRNA level and its regulatory functions.hsa-miR-206 also abolished the induction on LXR target gene SCD-1 by LXR ligand GW3965 treatment.Collectively,hsa-miR-206 negatively regulated LXRα mRNA level and its regulatory function.Our findings provides new insight to the regulation on endogenous LXRα by a novel mechanism,which implies potential interventions on LXRα related physiological functions.