25个油棕SSR标记的开发及应用这些标记评估油棕资源的遗传多样性

Development of 25 SSR Markers and Their Uses in Evaluating Genetic Diversity of Oil Palm Germplasm Resources

应用NCBI网站上公布的EST序列,挖掘SSR位点,并根据SSR位点的侧翼序列设计引物,对18份油棕资源的DNA进行了PCR扩增,扩增结果显示,在72对引物的PCR扩增中,25对引物具有多态性特征,并且其杂合度在0.1049和0.6605之间,平均杂合度为0.4702,这暗示了这些标记具有中度的多样性。同时,应用这些有多态性的标记评估了这些油棕资源的遗传多样性和群体结构,分析结构显示:椰子研究所油棕资源圃的遗传多样性最为丰富,此外,聚类分析表明不同位置来源的油棕资源展现了显著的遗传差异。本研究开发的SSR标记将能被应用到油棕的遗传育种研究中。

The research mined SSR loci according to EST sequences published in NCBI website, and designed primers based on the sequences flanking these SSR loci. Then, PCR amplification of the DNAs from 18 oil palm germplasm resources was carried out to test their diversity. The amplification results showed that： among the PCR amplification with 72 pairs of primers, that with 25 pairs of primers obtained polymorphic bands, and their heterozygosity varied from 0. 1049 to 0. 6605, with the average of 0. 4702, indicating these markers had an intermediate level of genetic variance. Meanwhile, these polymorphic SSR markers were used to e- valuate the genetic diversity and population structure of these oil palm germplasm resources. The results showed that the oil palm germplasm resources from Coconut Research Institute had the richest genetic diversity. Clustering analysis demonstrated that there was a significant genetic difterence among these oil palm resources from different regions. The developed SSR markers in this re- search will have an immediate application in the genetic breeding of oil palm.