芎蚓中风颗粒的质量标准研究

Study on quality standard for Xiongyin Zhongfeng granules

[目的]建立芎蚓中风颗粒的质量标准。【方法】采用薄层色谱法对其进行定性分析。采用高效液相色谱（HPLC）法测定其中阿魏酸和芍药苷的含量。阿魏酸：C18色谱柱（250mm×4．6mm，5μm）；甲醇-1％醋酸溶液（35：65）为流动相；流速为1mL／min；检测波长为321nm。芍药苷：C18色谱柱（250mm×4．6mm，5μm）；乙腈-0．01％磷酸溶液（15：85）为流动相；流速为1mL／min；检测波长为230nm。【结果】建立了该药中川芎、当归、地龙、赤芍的鉴别方法。阿魏酸在4．286～42．86μg／mL（r=1．000）线性关系良好，平均回收率为99．7％。RSD=2.8％（n=6）。芍药苷在0．05872-0．5872mg／mL（r=0．99999）线性关系良好，平均回收率为99．5％，RSD=0．67％（n=6）。【结论】该方法简便、可靠、重复性好，可用作芎蚓中风颗粒的质量控制。

[Objective] To establish the quality standard for Xiongyin Zhongfeng granules. [Methods] A identification o[ Xiongyin Zhongfeng Granules was periormed by TLC. The ferulic acid and paeoniflorin were determinated by HPLC. Ferulic acid： It was separated on （250mm×4.6mm,5μm）column. The mobile phase was methanol-1% acetic acid solution （35：65）. The flow rate was 1 mL/min. The detection wavelength was 321nm. Paeoniflorin： It was separated on C18 （250mm×4.6mm,5μm）column. The mobile phase was acetonitrile-0.01% phosphate solution （ 15：85 ）. The flow rate was 1 mL/min. The detection wavelength was 230 nm. [Results] Identification of ligusticum chuanxiong hort, angelica, pheretima,paeonia of Xiongyin Zhongfeng granules was established by TLC. The linear range of ferulic acid was 4.286-42.86 μg/mL （r=l.000）.The average recovery was 99.7% with RSD=2.8% （n=6）. The linear range of paeoniflorin was 0.058 72-0.587 2 mg/mL （r=0.999 99）.The average recovery was 99.5% with RSD=0.67% （n=6）.[Conclusion] These methods are simple, accurate and repeatable. They can be used as quality standard for Xiongyin Zhongfeng granules.