催化动力学光度法研究氧化乐果对碱性磷酸酶的抑制作用

Study on the Inhibition of Alkaline Phosphatase by Folimat with Catalytic- kinetic Spectrophotmetry

基于有机磷农药对碱性磷酸酶活性的抑制作用,以碱性磷酸酶(ALP)催化3-吲哚酚磷酸酯(3-IP)生成靛蓝为研究体系,利用催化动力学光度法研究了氧化乐果对ALP的抑制作用.结果表明,在pH 8.0Tris-HCl溶液中,靛蓝的最大吸收波长为683 nm,ALP的最佳浓度为0.15U/mL,3-IP的最佳浓度为3×10-3mol/L,氧化乐果对ALP的抑制时间为20 min.该研究为建立快速检测农药残留的催化动力学光度法奠定了基础,对构建有机磷农药抑制型生物传感器具有重要应用价值.

Based on the inhibition of Alkaline Phosphates （ALP） by organphosphorous pesticides,the catal ysis of 3 - Indoxyl Phosphate （3 - IP） by ALP to produce indigo blue was as the measurement system, and the in hibition of ALP by folimat was studied with catalytic kinetic speetrophotometry. The results of experiments show that the maximum absorption wavelength of indgo blue was 683 nm in Tris - HCI solution （pH8.0） ; the optimal concentration of ALP and 3 - IP was 0.15 U/mL and 0. 012 mmol/L respectively, and the inhibition time was 20 min. The research results above lay the foundation for the construction of catalytic - kinetic spectrophotmetry for pesticides residue determination and have important application for fabrication of biosensors based on organphos phorous pesticides - inhibition.