胶质源性神经营养因子对脊髓损伤后功能及前角运动神经元酶组织化学改变的研究

Effects of GDNF to Enzyme Histochemical Changes on Anterior Horn Motor Neurons Following Incomplete SpinalCord Injury in Rats

目的 探讨胶质细胞源性神经营养因子 (GDNF)对损伤脊髓运动功能及前角运动神经元酶组织化学改变的影响。方法 改良Allen撞击致T13 脊髓不完全损伤 ,蛛网膜下腔分别给予生理盐水和GDNF ,不同时间分别 :①测定大鼠后肢神经功能 ;②利用酶组织化学染色方法显示脊髓侧前角运动神经元中胆碱酯酶 (ChE)和酸性磷酸酶 (ACP)活性并通过计算机图像分析系统将酶活性量化、比较。结果 ①神经功能随时间延长而逐渐恢复 ,GDNF有助于功能恢复 ,但 3周时均未达正常标准 ;②ChE和ACP活性随时间延长而向正常趋近 ,GDNF显著加强了这一趋势 ;③随着ChE水平上升和ACP水平隆低 ,大鼠后肢运动功能逐渐恢复 ,两者呈现较强的相关性。结论 ①前角运动神经元酶学改变与神经功能恢复密切相关 ;②GDNF加强前角运动神经元酶学改变 ,呈现对损伤神经元有保护作用。

Objective ToinvestigateeffectsofGDNFtoenzymehistochemicalchangesonanteriorhormmotorneuronsfollowingincom pletespinalcordinjuryinrats.Methods Animalsweredividedintothreegroupsofnormalcontrast,NSandGDNFweresupplied throughsubarachnoidcavityafterT13segmentsofspinalcordwereinjuredbymodifiedAllen’scrushmethod ,thespecimenofspinal cordweretakenatdifferenttimeandsectioned .EnzymehistochemicaltechniqueofACPandChEwasconducted .TheactivityofACP andChEatfrontcornermotorneuronwerequantizedandcomparedapplyingimageanalysisofcomputer.Results Followingincompletespinalcordinjuryinrats,(1)Nervefunctionoflowerlimbsgotgradualrecoveryastimegoingon ,andGDNFenhancedthisfunction progression ,butitcouldnotreachnormalcriteriaafter3weeks;(2 )TheenzymeactivityofChEandACPatfrontcornermotorneuron tendedtonormalstandardgraduallyandGDNFcouldstrengthenobviouslythistrend ;(3)AsthelevelofChEincreasingandACPde creasingofanteriorhornneurons,behindlimbsofinjuriedratsgotrevoveredgradually,andthereisstrongcorrelationbetweentheactivi tyofChEandACPandratmotorfunction .Conclusion (1)Enzymechangesofspinalcordfronthornmotorneuronwerecorrelated closelywithmotorfunctionrestore;(2 )GDNFconsolidedthisenzymechangesandindicatedthatGDNFplaysaroleinprotectingthe spinalcordmotorneuronagainstinjury.