瑞香狼毒中黄酮类化合物的化学成分及其对朱砂叶螨触杀活性的研究

The Study on Chemical Structure of Flavonoids from Stellera chamaejasme L. and the Acaricidal Activity Against Tetranychus cinnabarinus B.

为研究瑞香狼毒(Stellera chamaejasme L.)中黄酮类化合物的杀螨活性。采用多种色谱技术分别对瑞香狼毒乙酸乙酯和正丁醇萃取物进行了分离纯化,利用核磁共振波谱技术对化合物进行了结构鉴定;采用玻片法进行了朱砂叶螨的活性测定。从乙酸乙酯萃取物中分离纯化得到2个化合物:分别鉴定为瑞香狼毒素A(1)、槲皮素(2);从正丁醇萃取物分离纯化得到1个黄酮类化合物:鉴定为(+)-表枇杷素(3);波片法显示:3个化合物对朱砂叶螨(Tetranychus cinnabarinus B.)触杀活性的半致死浓度(LC50)值分别为4.9434、7.6930、2.2494 mg/mL。瑞香狼毒素A、槲皮素和(+)-表枇杷素均具有一定的杀螨活性,其中表枇杷素杀螨活性最强。

To study acaricidal activity of compounds from Stellera chamaejasme L.. Flavonoids were isolated and purified from EtOAc and n-butanol extract of S. chamaejasme L. respectively by using various chromatographic techniques, and their chemical structure were identified on basis of NMR data. Forthermore, their acaricidal activity tests were chected out. The three flavonoids were identified as daphnelantoxin A （1）, quercetin （2） and （＋）-epiafzelechin （3） comparing with literature. The half lethal concentration （LC50） values of their touch position against Tetranychus cinnabarinus B. were detected as 4.9434 mg/mL, 7.6930 mg/mL, 2.2494 mg/mL, respectively. The result showed that epiafzelechin had better~ acaricidal activity.