甲醛及孔雀石绿对哲罗鲑发眼卵MHC及IL-1β含量的影响

Effects of Formaldehyde and Malachite Green on MHC and IL-1β Content in Eyed Eggs of Taimen (Hucho taimen Pallas.)

为了研究甲醛及孔雀石绿消毒对哲罗鲑发眼卵主要组织相容性复合体(MHC)及白细胞介素(IL-1β)含量的影响。将哲罗鲑发眼卵随机分为2组,每组设3个平行,每个平行3000粒卵,按照500 mg/kg和2 mg/kg浓度对鱼卵进行甲醛和孔雀石绿浸泡消毒,每天1次,每次持续30 min,对消毒前、后30 min采集的样品进行MHC及IL-1β含量测定。结果表明:甲醛及孔雀石绿消毒均对哲罗鲑受精卵中MHC和IL-1β含量造成了一定影响。甲醛消毒促使卵内MHC含量稳步提升,而对IL-1β含量无明显影响;而孔雀石绿消毒导致卵内MHC产生无序剧烈变化,且IL-1β含量急速提升,可能已对卵造成了炎症反应。甲醛消毒对卵免疫系统产生的影响明显小于孔雀石绿且无明显不良反应,可用于哲罗鲑鱼卵日常消毒使用。

Influence of Formaldehyde and Malachite Green on MHC and IL-1β content in Eyed Eggs of Taimen （Hueho taimen Pallas.） was studied in this paper. Eyed eggs of Hueho taimen was randomly divided into 2 groups （n=3000）, with each group subdivided into 3 replicates. Formaldehyde and malachite green were used to disinfect the eyed eggs by immersion method at the concentration of 500 mg/kg and 2 mg/kg respectively. Disinfection was performed continuously for 30 min once per day and the samples were taken at half an hour before and after disinfection. MHC and IL-1β content of samples were then determined. The results indicated that both formaldehyde and malachite green caused a certain influence on MHC and IL-1β contents in eyed eggs of Hucho taimen. Disinfection with formaldehyde steadily improved the content of MHC in eggs while had no obvious effects on IL-1β content. However, disinfection with malachite green led a disordered and violent change to MHC content in eggs and could increase rapidly the IL-1β content, which showed that disinfectants maybe have produced inflammation on eggs. The influence of formaldehyde on eggs was obviously lower than that of malachite green and there was no obvious bad effect, which indicated that formaldehyde could be applied to disinfection of eyed eggs in Hueho taimen.