磷化氢熏蒸对桔小实蝇氧化代谢体系的影响研究

The Effect of Phosphine Fumigation on Oxidative Metabolism of Bactrocera dorsalis Hendel

为阐明磷化氢的生化毒理机制,以重要检疫性害虫桔小实蝇(Bactrocera dorsalis Hendel.)为试虫,研究了25℃下磷化氢熏蒸对桔小实蝇3龄幼虫的毒力以及磷化氢熏蒸不同时间对桔小实蝇3龄幼虫氧化代谢体系的影响。结果显示:(1)25℃条件下磷化氢熏蒸桔小实蝇3龄幼虫48 h的LD50、LD90、LD99分别为0.28、0.37、0.46 mg/L;(2)磷化氢熏蒸抑制了桔小实蝇的呼吸作用;(3)随熏蒸时间延长,桔小实蝇体内过氧化氢含量和脂质过氧化水平不断上升,氧化压力逐渐增加;(4)磷化氢抑制了过氧化氢酶活性,最大抑制率为55.99%,增强了过氧化物酶活性、超氧化物歧化酶活性及抑制羟自由基能力,最大提高率分别为141.48%、130.21%、155.90%。本研究确认了磷化氢熏蒸杀灭桔小实蝇的有效性,其作用机制可能是通过抑制过氧化氢酶活性进而加剧活性氧积累实现的。

To clarify the biochemical mechanism of phosphine toxicology,studies were undertaken to establish the effects of phosphine fumigation on the mortality and oxidative metabolism in 3rd instars of Bactrocera dorsalis Hendel. under 25℃. The results showed that：（1） the dosages to achieve 50%,90% and 99% mortality were 0.28,0.37,0.46 mg/L respectively in 48 h fumigation at 25℃;（2） the respiration of B. dorsalis was consistently inhibited during the fumigation;（3） phosphine inhibited catalase activity with maximum inhibition rate 55.99%,increased peroxidase and superoxide dismutase activity and inhibition of hydroxyl radical capacity with the greatest increasing rates of 141.48%,130.21%,155.90% respectively. These results confirmed the toxicity of phosphine against B. dorsalis,and verified that accumulation of reactive oxygen species was one possible mechanism of phosphine toxicology by inhibiting the activity of catalase.