1株酵母菌产GABA发酵条件的优化

Optimization of Fermentation Conditions for Producing γ-Aminobutyric Acid (GABA) by Candida parapsilosis GPT-5-11

通过液态发酵试验研究专利菌株近平滑假丝酵母(Candida parapsilosis)GPT-5-11(专利号:201010182948.5)合成γ-氨基丁酸的最佳工艺条件。对发酵培养基及发酵培养条件(初始pH、添加L-MSG浓度和发酵周期)进行优化。结果表明,最佳培养基成分为:葡萄糖24.5g/L,酵母膏6.2g/L,大豆蛋白胨6.2g/L,吐温80 2 mL,乙醇4 mL,MgSO4·7H2O 0.2 g/L,MnSO4·4H2O 0.045 g/L,L-MSG 12.5 g/L,含0.5%谷氨酸;培养基中添加L-MSG浓度为12 g/L;pH为6.5;37℃发酵48 h。在最优条件下,γ-氨基丁酸产量最高可达2.58 g/L。实验为该菌株的进一步利用提供了参考依据。

The optimized fermentation conditions of Candida parapsilosis GPT-5-11（patent number：201010182948.5） were analyzed for the production ofγ-aminobutyric acid by liquid fermentation experiments.The liquid fermentation experiments included selection of fermentation medium and cultivating conditions such as pH,concentration of L-MSG and period. The results showed that the optimum conditions were as follows：the optimal medium composition was glucose 24.5 g/L,yeast 6.2 g/L,soy peptone 6.2 g/L,Twain80 2 g/L mL,alcohol 4 mL,MgSO4·7H2O 0.2 g/L,MnSO4·4H2O 0.045 g/L,L-MSG 12.5 g/L; concentration of L-MSG was 12 g/L; cultivating pH was 6.5; and cultivating period was 48 h under 37℃. Under optimal conditions,γ-aminobutyric acid production reached 2.58 g/L. It provided the experimental data for application of GPT-5-11.