激活剂对油藏微生物群落及其驱油能力的影响

Effect of activator on community structure and oil displacement ability of reservoir microbial

以胜利油田油藏微生物群落为对象,利用物理模拟及末端限制性片段长度多态性(TRFLP)技术研究了不同激活剂调控下油藏微生物群落结构与其驱油能力之间的关系.研究发现:在辅助补充空气的条件下,随着激活剂的加入,微生物多样性减少;分支杆菌属(Mycobacterium)、沼气甲基产孢弧菌属(Methylosporovibrio)、热微菌属(Thermomicrobium)、甲基单胞菌属(Methylomonas)、乳杆菌属(Lactobacillus)等优势种属消失,芽胞杆菌属(Bacillus)、嗜甲基菌属(Methylophilus)、假单胞菌属(Pseudomonas)、梭菌属(Clostridium)和栖热菌属(Thermus)等构成了激活后的优势菌群;不同的激活剂所对应的岩心产出液中微生物群落结构不同,提高采收率的幅度也不同.分析表明,栖热菌属(Thermus)是提高原油采收率的关键种群,产出液中含有该菌属的2个岩心的采收率分别提高了8.2%和7.8%.

Taking some oil reservoir in Shengli Oilfield as an example,the relationship between the community structure and oil displacement ability of reservoir microbial under the effect of different activators is studied by means of physical simulation and terminal restriction fragment length polymorphism analysis( T-RFLP). The results show that,under the supplementary air,the species of the reservoir microbial reduce after activator is added to the reservoir; the original predominant microbial species of Mycobacterium,Methylosporovibrio,Thermomicrobium,Methylomonas and Lactobacillus are not be detected,and Bacillus,Methylophilus,Pseudomonas,Clostridium and Thermus become predominant species. In addition,the oil displacement efficiency and the community structure of the microbial are different when different nutrients are injected to the reservoir. Thermus is the key genus to enhancing oil recovery factor. The oil recovery factor of two cores whose produced fluid contains Thermus is enhanced by 8. 2% and 7. 8% respectively.