固体发酵裂褶多糖免疫调节及抗肿瘤活性研究

Immunity-modulatory and Antitumor Activities of Schizophyllan Produced with Solid-state Fermentation

以麦麸和玉米芯为原料固体发酵生产裂褶多糖（schizophyllan,SPG）,采用辐照技术降解处理SPG（irradiated schizophyllan,ISPG）,研究辐照前后裂褶多糖分子量、体内免疫调节及抗肿瘤活性变化。结果显示,辐照能有效降低天然SPG分子量,分子量从106~107Da（SPG）降低到104~105Da（ISPG）。SPG和ISPG均具有良好免疫调节和抗肿瘤活性,SPG和ISPG中剂量（100 mg/kg体重·d）和低剂量（50 mg/kg体重·d）组均对S180荷瘤小鼠羊红细胞诱导迟发型变态反应（DTH）和脾细胞介导羊红细胞定量溶血值（QHS）具有显著增强功效,ISGP中剂量组（100 mg/kg体重·d）能显著增强小鼠巨噬细胞吞噬鸡红细胞效果,提升小鼠非特异性免疫功能;SPG和ISPG不同剂量组抑瘤率均在45%以上,ISPG中剂量组（100 mg/kg体重·d）抑瘤率最高（74.80%±6.83%）。总体看来,辐照处理后的ISPG比天然SPG具有更好的免疫调节及抗肿瘤活性。

Schizophyllan（ SPG） was produced by the fungus Schizophyllum commune under solid-state fermentation conditions in this study. Irradiation technology was used to treat SPG and molecular weight and biological effects of SPG and irradiated schizophyllan（ ISPG） on the immunity-modulatory and antitumor activities in S180 tumor mice models in vivo were investigated. Irradiation technology decreased significantly the molecular weight of SPG（ P 0. 05）,and the range of molecular weight of SPG and ISPG were 106~ 107Da and 104~ 105Da,respectively. Both SPG and ISPG with medium dose（ 100 mg / kg） and low dose（ 50 mg/kg） had potent immune-modulatory effects in S180 tumor mice model and exhibited significant effect on delayed-type hypersensitivity（ DTH） response and quantitative hemolysis spectrophotometry（ QHS） test（ P 0. 05）. Besides,ISPG with low dose（ 50 mg / kg） elevated significantly the phagocytosis of macrophage（ P 0. 05）. The results still showed that SPG and ISPG had great antitumor effects,and ISPG at medium dose（ 100 mg / kg） had maximum inhibition rate（ 74. 80% ± 6. 83%）. Therefore,ISPG had better immunity-modulatory and antitumor activities than natural SPG.