正常和缺氧条件下磁场对人脑微血管内皮细胞增殖的影响

Effect of magnetic fields on human brain microvascular endothelial cell proliferation under normal and hypoxia conditions

[目的]观察不同强度的永磁磁场对正常和缺氧条件下人脑微血管内皮细胞(HBMEC)增殖的影响。[方法]采用正常和缺氧两种条件体外培养HBMEC,实验分为10组,即对照组、药物组(银杏叶注射液350 mg/L)、不同强度磁场组(3.4、5.6、13.8、27.8、52.6、107.0、178.9、292.2 mT),各组细胞于磁场作用48 h后收集样本,用四甲基偶氮唑盐法(MTT法)检测细胞增殖。[结果]正常和缺氧条件下,与对照组相比较,药物组有统计学意义(P<0.05),各磁场组差异均无统计学意义(P>0.05);磁场组组间比较,差异无统计学意义(P>0.05)。[结论]正常和缺氧条件下,磁感强度3.4、5.6、13.8、27.8、52.6、107.0、178.9、292.2 mT的磁场对人脑微血管内皮细胞的增殖无影响。

[Objective] To observe effect of different permanent magnetic fields on human brain microvascular endothelial cell （HBMEC） proliferation under normal and hypoxia conditions. [Methods] HBMEC were cultured in vitro under normal condition and hypoxia condition, experimental cells were divided into 10 groups, control group, ginkgo biloba injection（350 rag/L） group, different induction magnetic field groups （3.4, 5.6, 13.8, 27.8, 52.6, 107.0, 178.9, 292.2 mT）, each group was collected specimens 48 h after planking, MTT method was used to measure cell proliferation. [Results] Compared with control group, the drug group was statistically different （P〈0.05） under normal condition and hypoxia condition, magnetic field groups were no statistically different （P〉0.05）; there was no statistical difference between magnetic field groups （P〉0.05）. [Conclusion] Magnetic field groups with magnetic induction 3.4,5.6,13.8,27.8,52.6,107.0,178.9,292.2 mT have no effect on proliferation of HBMEC cultured under normal or hypoxia condition.