9.4TMR波谱检测间充质干细胞成骨分化代谢特征的研究

9.4 T MR spectroscopy detection of mesenchymal stem cells into osteoblasts metabolism characteristics

目的研究间充质干细胞向成骨细胞分化的高分辨MR波谱(MRS)代谢特征。材料与方法培养获取间胎儿脐带充质干细胞,建立体外诱导间充质干细胞向成骨细胞分化的模型,利用甲醇/氯仿获取细胞代谢提取物,经后处理后利用9.4 T高分辨率MRS检测代谢提取物频谱特征。结果诱导间充质干细胞向成骨细胞分化前后,位于2.02 ppm及2.25 ppm的脂质峰明显减低,且在1.77 ppm出现一新的代谢峰,该代谢峰可能为亮氨酸。结论间充质干细胞成骨分化后MRS具有一定特征,位于1.77 ppm的代谢峰可能做为间充质干细胞成骨分化的生物学标识。

Objective： To explore the metabolite profile of mesenchymal stem cells （MSCs） underwent osteogenic differentiation using 9.4 T high resolution MR spectroscopy. Materials and Methods： Human umbilical cord mesenchymal stem cells were cultured and collected. MSCs were induced osteogenic differentiation for 2 weeks and cell metabolite extractions were prepared by methanol-chlorotbrm （M/ C） method. Cell extractions were then analyzed on a 9.4 T MRS device. Results： After osteogenic inducement, the fatty acid metabolite peak at 2.02 ppm and 2.25 ppm obviously decreased, and there appeared a new metabolite peak at about 1.77 ppm, which is most probably representative of leucine. Conclusions： There are some specific characteristics on MRS of MSCs new arised peak at 1.77 ppm may serve as osteogenic differentiation. underwent osteogenic differentiation. The the biomarker for mesenchymal stem cells