RP-HPLC测定尼麦角林缓释片的含量

Determination of the content of nicergoline in release tablets by RP-HPLC

目的:建立尼麦角林缓释片的含量测定方法。方法:采用RP-HPLC法,以Agilent ZORBAX C8(250mm×4.6mm,5μm)为色谱柱;乙腈-0.05mol/L磷酸盐缓冲液(42∶58)为流动相;检测波长280nm。结果:尼麦角林在0.243～0.973μg范围内,与峰面积呈良好的线性关系(γ=1.0000);平均回收率为99.8%,RSD为0.64%(n=6)。结论:该法快速、简便、重现性好,可用于尼麦角林缓释片的质量控制。

Objective：To establish a method for determination of contents in Nicergoline Release Tablets. Methods：An RP-HPLC method was used with Agilent ZORBAX C8（250mm×4.6mm,5μm）column;the mobile phase consisted of acetonitrile-0.05mol/L phosphate buffer（42∶58）and UV detection at 280 nm. Results：Nicergoline showed good linear relationship in the range of 0.243~0.973μg（γ =1.0000）. The average recovery was 99.8%,RSD was 0.64%（n =6）. Conclusions：This method is rapid,simple and reproducible,The method can be used for the quality control of nicergoline release tablets.