密闭性中药复合敷料的提取工艺优选

Optimization of Extraction Process of Airtight Chinese Medicine Composite Dressings

目的： 优选密闭性中药复合敷料的提取工艺。方法： 以人参皂苷Rg1，Rb1含量和浸膏得率为评价指标，通过单因素试验优选提取次数，利用正交试验考察乙醇体积分数、乙醇用量、提取时间对密闭性中药复合敷料提取工艺的影响。采用HPLC测定人参皂苷Rg1，Rb1含量，流动相水（A）-乙腈（B）梯度洗脱（0-10 min，81% A；10-38 min，81%-79% A；38-90 min，79%-66% A），检测波长203 nm。结果： 各因素对提取工艺的影响顺序为乙醇体积分数〉提取时间〉乙醇用量。最佳提取工艺条件为加8倍量70%乙醇提取2次，每次2 h；平均浸膏得率25.45%，人参皂苷Rg1，Rb1平均质量浓度分别为0.46，0.37 g·L-1。结论： 建立的含量测定方法准确、重复性好，优选的提取工艺稳定、合理、可行，为民间外伤验方的二次开发提供参考。

Objective： To optimize extraction process of airtight Chinese medicine composite dressings. Method： With contents of ginsenoside Rg1 and Rb1,extract yield as evaluation indexes,extraction times was optimized by single factor test,orthogonal design was adopted to optimize extraction technology of airtight Chinese medicine composite dressings by taking the amount and content of ethanol,extraction time as factors.Contents of ginsenoside Rg1 and Rb1 were determined by HPLC,mobile phase of water（A）-acetonitrile（B） with a gradient elution program（0-10 min,81% A;10-38 min,81%-79% A;38-90 min,79%-66% A）,detection wavelength 203 nm. Result： Effect of each factor on extraction technology was in order of ethanol concentration〉extraction time〉ethanol amount.Optimum extraction process were as follows：extracted 2 times with 8-fold the amount of 70% ethanol,2 hours per time;Average extract yield was 25.45%,average concentration of ginsenoside Rg1 and Rb1 were 0.46,0.37 g·L-1. Conclusion： This established method was accurate and reproducible,optimized extraction process was stable,reasonable and practicable,this study could provide a reference for secondary development of clinical prescriptions.