HPLC测定复方肝炎颗粒中槲皮素和山奈素的含量

Determination of Content of Quercetin and Kaempferol in Compound Ganyan Granules by HPLC

目的： 高效液相法测定复方肝炎颗粒中槲皮素和山奈素的含量。方法： 采用HPLC，样品经80%甲醇超声、酸水解后，采用色谱柱C18 （4.6 mm× 250 mm，5 μm），甲醇-0.4%磷酸（50：50）为流动相，流速1.0 mL·min-1，检测波长360 nm，柱温30℃。结果： 槲皮素在0.050 1-0.501 μg（r=0.999 9）和山奈素在0.024 2-0.242 μg（r=0.999 5）均呈良好线性关系；槲皮素和山奈素加样回收率分别为99.69%（RSD 0.22%），99.30%（RSD 0.82%）（n=9）。结论： 该法操作简便﹑结果准确、重复性好﹑结果准确可靠，可用于复方肝炎颗粒的质量控制要求。

Objective： To establish a HPLC method for quantification of quercetin and kaempferol in compound Ganyan granules. Method： Samples were extracted in 80% methanol with ultrasonic process and hydrolyzed for 1 h. The HPLC was performed on an ODS-C18column （4.6 mm×250 mm, 5 μm） with methanol-0.4% H3PO4 （50：50） as mobile phase. The flow rate was 1.0 mL·min-1 and the column tempreture was kept at 30℃. The eluate was detected at 360 nm. Result： The linear ranges of quercetin and kaempferol were 0.050 1-0.501 μg （r=0.999 9） and 0.024 2-0.242 μg （r=0.999 5） respectively. The average recoveries of quercetin and kaempferol were 99.69%（RSD 0.22%） and 99.30%（RSD 0.82%）. Conclusion： The method can be used for quality control of Ganyan granules. It is simple, rapid and accurate for simultancous determination of two constituents in compound Ganyan granules.