珠芽景天抗肿瘤活性研究及槲皮素和山柰素的含量测定

Determination of Quercetin and kaempferol in Sedum bulbiferum and Research on Its Anti-tumor Activities

目的： 研究珠芽景天的有效部位及其体外抗肿瘤作用，并建立其槲皮素与山柰素的含量测定方法。方法： 采用MTT法检测珠芽景天的乙酸乙酯、正丁醇提取部位及总黄酮提取物对人肝癌细胞株HepG2、人食管癌细胞株EC109、人结肠癌细胞株SW480的体外增殖抑制作用；采用HPLC测定珠芽景天中槲皮素与山柰素的含量。结果： 珠芽景天的乙酸乙酯提取部位及总黄酮提取物对3种癌细胞株都有明显抑制作用，其作用都随着质量浓度的增加而增强；槲皮素、山柰素进样量分别在0.03-0.36，0.08-0.96 μg与峰面积呈良好的线性关系（r=0.999 9），平均加样回收率分别为98.90%，98.27%，RSD均〈1.70%（n=6）。结论： 珠芽景天具有较显著的体外抗肿瘤作用；建立的HPLC含量测定方法准确可靠、重复性好，可用作珠芽景天有关活性物质含量控制的参考依据。

Objective： To investigate the anticancer effective part and anticancer effect of Sedum bulbiferum in vitro,and to establish a method for determination of quercetin and kaempferol in S. bulbiferum by HPLC. Method： The inhibitory activities of the the different extract fractions and the total flavones of S. bulbiferum on cancer cells proliferation of liver,esophageal and colon （HepG2, EC109, SW480）were tested by MTT assay.HPLC method for the determination of quercetin and kaempferol in S. bulbiferum was established. Result： The growth and proliferation of the 3 cancer cells were all significantly inhibited by ethyl acetate fractions and the total flavonoids of S. bulbiferum. With the increase of the concentration, inhibiting action increased. The linear ranges of quercetin and kaempferol were 0.03-0.36 μg （r=0.999 9） and 0.08-0.96 μg （r=0.999 9） respectively;their average recoveries were 98.90%（RSD 1.15%） and 98.27%（RSD 1.70%）respectively. Conclusion： S. bulbiferum has significant effect of anti-tumor in vitro. The method of HPLC is accurate,reproducible and could be used for active ingredient content determination of this crude drug.