铁包金根、茎特征图谱的研究

HPLC Characteristic Fingerprint for Roots and Stems of Berchem lineata

目的:建立铁包金根、茎HPLC特征图谱,为该药材质量评价研究提供参考。方法:采用HPLC构建24批不同产地不同种类铁包金根、茎的特征图谱,通过聚类分析和主成分分析对特征图谱进行化学模式识别。结果:以11个共有峰为特征指纹信息构建HPLC特征图谱,9批细叶勾儿茶和多叶勾儿茶根为一类,12个细叶勾儿茶和多叶勾儿茶的茎和3个多叶勾儿茶的根聚为一类。结论:HPLC特征图谱的构建为铁包金根、茎的质量评价研究提供参考依据。

Objective： To establish a HPLC characteristic fingerprints for the determination of the roots and stems of Berchem lineata. Method： The characteristic fingerprints of 24 batches of roots and stems of B. Lineata and B. floribunda, which were belong to different kinds and collected from different places, were established by HPLC. A chemical pattern recognition method was constructed by using cluster analysis and principal components analysis （PCA） method. Result： 11 common peaks were found in the HPLC characteristic fingerprints. 24 samples were divided into two branches. 9 batches of roots of B. Lineata and B. floribunda clustered to a clade. 3 roots of B. floribunda, 12 stems of B. Lineata and B. floribunda were first clustered together as a sister clade to roots of B. Lineata and B. floribunda. Conclusion： The established method can be used for quality control of the roots and stems of B. lineata.