舒胸胶囊微粉对血瘀大鼠全血黏度及血小板活性的影响

Effects of Shuxiong Capsule Micropowder on Whole Blood Viscosity and Platelet Activity of Rats with Acute Blood Stasis

目的:研究舒胸胶囊微粉对急性血瘀大鼠全血黏度和血浆α颗粒膜蛋白/alpha-granular membrane protein(GMP-140)、血栓调节蛋白/thrombomodulin(TM)含量的影响。方法:60只大鼠随机分为舒胸胶囊微粉0.094 5,0.283 5 g·kg-1组、舒胸胶囊0.094 5,0.283 5 g·kg-1组及空白对照组和模型对照组。ig给药,每日1次,连续给药14 d后,除空白对照组外各组均采用肾上腺素加冰浴的方法复制急性血瘀模型,次日腹主动脉取血,肝素抗凝,检测大鼠全血黏度、血浆GMP-140及TM含量。结果:与空白对照组比较,模型对照组大鼠全血低切、中切、高切黏度及血浆GMP-140及TM含量显著升高;与模型对照组比较,舒胸胶囊微粉、舒胸胶囊均可降低大鼠全血黏度及血浆GMP-140及TM含量,以舒胸胶囊微粉高剂量组疗效最佳。结论:舒胸胶囊微粉能够改善急性血瘀大鼠血液流变性,具有明显的活血化瘀作用,其作用机制与降低大鼠血浆GMP-140及TM含量有关。

Objective： To study the effect of Shuxiong capsule micropowder on whole blood viscosity and the alpha-granular membrane protein（GMP-140） as well as thrombomodulin（TM） content of blood plasma of rats with acute blood stasis. Methold：Sixty rats were randomly divided into six groups as following：the group of Shuxiong capsule micropowder 0.094 5 g·kg-1, the group of Shuxiong capsule micropowder 0.283 5 g·kg-1, the group of shuxiong capsule 0.094 5 g·kg-1, the group of shuxiong capsule 0.283 5 g·kg-1 as well as blank and model groups. After administration for 14 days continuously, acute blood stasis models were replicated by means of epinephrine in ice-bath. Then the effects of shuxiong capsule micropowder on whole blood viscosity and the GMP-140 as well as TM content of blood plasma of model rats were observed. Result： When compared with blank group, the model rats＇ whole blood low, moderate and high cuts viscosity as well as the GMP-140 and TM content of blood plasma all rise remarkably.Compared with model group, the Shuxiong capsule micropowder and Shuxiong capsule can decreased whole blood viscosity,GMP-140 and TM content of blood plasma. The group of Shuxiong capsule micropowder 0.283 5 g·kg-1 had better effect. Conclusion： The results stated that Shuxiong capsule micropowder can improve blood rheology behavior of rats with acute blood stasis and has remarkable function of activating blood circulation and eliminating stasis. The mechanism might be related to the reduction of the GMP-140 and TM content of blood plasma.