连翘酯苷对PC12细胞增殖及其细胞损伤模型的保护作用

Neuroprotective Effects of Forsythiaside on Glutamate,Low-glucose and Low Serum,Aβ_(25～35)-induced Neurotoxicity in PC 12 Cell

目的： 研究连翘酯苷对PC12细胞增殖及其损伤模型的影响。方法： 应用谷氨酸（20 mmol·L-1）、低糖低血清培养基及β淀粉样蛋白25~35（Aβ25~35，10 μmol·L-1）建立谷氨酸、低糖低血清及Aβ25~35致PC12细胞损伤模型，观察连翘酯苷低、中、高浓度（0.1，1，5 μmol·L-1）剂量对上述模型细胞存活率的影响，同时观察连翘酯苷低、中剂量对Aβ25~35致PC12细胞凋亡的影响。结果： 连翘酯苷可提高PC12细胞存活率，提高谷氨酸、低糖低血清及Aβ25~35致PC12细胞损伤模型的存活率，与模型组比较，有显著性差异（P〈0.05），并呈现一定量效关系。连翘酯苷（0.1，1 μmol·L-1）可降低Aβ25~35引起PC12细胞凋亡率（P〈0.05，P〈0.01）。结论： 连翘酯苷在体外实验具有神经细胞保护作用。

Objective： To investigate the neuroprotective effects of forsythiaside on glutamate, low-glucose and low-serum, amyloid beta25~35 （Aβ25~35））-induced neurotoxicity in PC12 cell. Method： The glutamate （20 mmol·L-1）, low-glucose and low serum and Aβ25-35 induced （10 μmol·L-1） neurotoxicity models in PC12 cell were established and the neuroprotective effects of forsythiaside （0.1,1,5 μmol·L-1）on above models were observed. Result： Forsythiaside could improve the proliferation on PC12 cell and significantly reduced cell death induced by glutamate, low-glucose and low-serum and Aβ25-35.In addition, forsythiaside （0.1,1 μmol·L-1） significantly inhibited cell apoptosis induced by Aβ25-35. Conclusion： forsythiaside has neuroprotective effects on glutamate, low-glucose and low-serum and Aβ25-35-induced neurotoxicity in PC12 cell.