摘要
目的:研究淫羊藿苷对体外培养大鼠股骨组织(骨干和骨骺端)吸收活性的影响。方法:体外分离培养大鼠股骨组织的骨干和骨骺端,48 h后采用终浓度为1×10-5mol·L-1的淫羊藿苷对体外培养骨干和骨骺端进行处理。测定抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,StrACP)活性;测定培养基中葡萄糖(glucose,Glu)和乳酸(lactic acid,Lac);RealTime RT-PCR检测抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,StrACP)、集落刺激因子(macrophage colony stimulating factor,MCSF),组织激酶K(cathepsin K,CTSK)mRNA表达水平。结果:1×10-5mol·L-1淫羊藿苷可抑制StrACP活性,增加培养基中乳酸含量和减少培养基中葡萄糖含量,抑制StrACP,MCSF,CTSK mRNA的表达水平。结论:淫羊藿苷抑制体外培养大鼠股骨组织的骨骺端和骨干吸收活性。
Objective: To investigate effect of icariin on femoral tissue(diaphyses and metaphysis) resorption and metabolic activity of rats in vitro. Method: The femoral tissue (diaphyses and metaphysis) was isolated which was treated with final concentration of 1×10-5 mol·L-1icariin in culture medium in vitro.The tartrate-resistant acid phosphatase (StrACP) activity, glucose and lactic acid content in culture medium were determined,StrACP, MCSF, CTSK mRNA expression was detected by Real-Time RT-PCR. Result: 1×10-5 mol·L-1 icariin inhibited the activity of StrACP, increased the content of lactic acid and decreased glucose content,and improved StrACP, MCSF, CTSK mRNA expression level. Conclusion: Icariin are adjust femurs tissue metabolic activity and inhibited resorption.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第24期201-205,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
甘肃省科技重大专项项目(09ZNKDA025))
国家自然科学基金项目(81270963)