摘要
目的:研究淫羊藿苷对体外培养大鼠股骨组织(骨干和骨骺端)吸收活性的影响。方法:体外分离培养大鼠股骨组织的骨干和骨骺端,48 h后采用终浓度为1×10-5mol·L-1的淫羊藿苷对体外培养骨干和骨骺端进行处理。测定抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,StrACP)活性;测定培养基中葡萄糖(glucose,Glu)和乳酸(lactic acid,Lac);RealTime RT-PCR检测抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,StrACP)、集落刺激因子(macrophage colony stimulating factor,MCSF),组织激酶K(cathepsin K,CTSK)mRNA表达水平。结果:1×10-5mol·L-1淫羊藿苷可抑制StrACP活性,增加培养基中乳酸含量和减少培养基中葡萄糖含量,抑制StrACP,MCSF,CTSK mRNA的表达水平。结论:淫羊藿苷抑制体外培养大鼠股骨组织的骨骺端和骨干吸收活性。
Objective： To investigate effect of icariin on femoral tissue（diaphyses and metaphysis） resorption and metabolic activity of rats in vitro. Method： The femoral tissue （diaphyses and metaphysis） was isolated which was treated with final concentration of 1×10-5 mol·L-1icariin in culture medium in vitro.The tartrate-resistant acid phosphatase （StrACP） activity, glucose and lactic acid content in culture medium were determined,StrACP, MCSF, CTSK mRNA expression was detected by Real-Time RT-PCR. Result： 1×10-5 mol·L-1 icariin inhibited the activity of StrACP, increased the content of lactic acid and decreased glucose content,and improved StrACP, MCSF, CTSK mRNA expression level. Conclusion： Icariin are adjust femurs tissue metabolic activity and inhibited resorption.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第24期201-205,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
甘肃省科技重大专项项目(09ZNKDA025))
国家自然科学基金项目(81270963)
