补肾利湿法对急性痛风性关节炎大鼠VEGF表达水平的影响

Effect of Bushen Lishi Methods on Expression of VEGF in Acute Gouty Arthritis Rats

目的:观察补肾利湿法对痛风性关节炎大鼠血管内皮生长因子(VEGF)表达水平的影响,探讨补肾利湿法防治急性痛风性关节炎(GA)的作用机制。方法:将70只Wistar大鼠随机分为7组:空白组、模型组、中药对照组、西药对照组及中药低、中、高剂量组,每组10只。采用尿酸钠联合氧嗪酸钾诱导大鼠急性痛风性关节炎模型,应用ELISA法,分别检测各组大鼠血清VEGF的含量,并对关节滑膜组织进行病理组织学检查。结果:7组VEGF水平差异均有统计学意义(P<0.01),模型组VEGF含量明显高于空白组(P<0.01);中药对照组、西药对照组和中药低、中、高剂量组VEGF含量均较模型组低(P<0.01),其中,西药对照组和中药中剂量组效果更明显;中药中剂量组与中药对照组、中药低剂量组、中药高剂量组相比有显著性差异(P<0.01),但与西药对照组相比无显著性差异。结论:补肾利湿法处方可抑制急性痛风性关节炎大鼠VEGF的表达,对防治痛风性关节炎具有一定作用。

Objective： To observe the effect of Bushen Lishi methods on expression of VEGF（vascular endothelial growth factor） in acute gouty arthritis rats and to explore the mechanism of Bushen Lishi methods in the treatment of GA. Method： Seventy rats were randomly divided into seven groups：blank control group, model group, herbal control group, western medicine control group, high dosage herbal group, medium dosage herbal group and low dosage herbal group, 10 rats in each group. The uric acid sodium and oxygen polybenzoxazines potassium were used to induce rat acute gouty arthritis model. ELISA was used to detect the concentration of serum VEGF in rats and analyze the data. Histopathologic examination of synovial tissue was also performed. Result： The differences of VEGF level between 7 groups were significant （P〈0.01）. The expression of VEGF in model group was higher than that in blank group, and the difference was significant （P〈0.01）. The expression of VEGF in model group was also apparently higher than that in herbal control group, western medicine control group, high dosage herbal group, medium dosage herbal group and low dosage herbal group （P〈0.01）, especially the effect of western medicine control group and medium dosage herbal group was more obvious. Conclusion： Bushen Lishi methods can inhibit the expression of VEGF in acute gouty arthritis rats. It has certain curative effect gouty arthritis.