黑水缬草石油醚部位改善小鼠睡眠作用及相关机制研究

Studies on Improving Sleep Function and Relative Mechanism of Mice by Petroleum Extract of Valeriana amurensis

目的:研究黑水缬草石油醚部位对小鼠睡眠的改善作用,考察其对小鼠脑内神经递质γ-氨基丁酸(GABA)及5-羟色胺(5-HT)含量的影响,初步探讨其改善睡眠的作用机制。方法:采用戊巴比妥钠协同作用实验,考察黑水缬草石油醚部位不同剂量对小鼠睡眠潜伏期和睡眠时间的影响以及联合氟马西尼注射液、5-羟色胺酸(5-HTP)和对氯苯丙氨酸(PCPA)对小鼠睡眠潜伏期和睡眠时间的影响;采用ELISA法测定小鼠脑组织GABA,5-HT含量,以确定黑水缬草石油醚部位改善睡眠的作用机制。结果:黑水缬草石油醚部位3个剂量(30,15,7.5 g·kg-1)均具有改善小鼠睡眠的作用,主要表现为缩短睡眠潜伏期和延长睡眠时间。联合氟马西尼注射液,5-HTP和PCPA后,对小鼠的睡眠时间均产生了明显的影响:与空白组比较,联合氟马西尼注射液可阻断黑水缬草石油醚部位和地西泮与戊巴比妥钠的协同作用。联合5-HTP可增强黑水缬草石油醚部位与戊巴比妥钠的协同作用:联合PCPA可阻断黑水缬草石油醚部位与戊巴比妥钠的协同作用。黑水缬草石油醚部位不同剂量均能使小鼠脑组织的神经递质GABA,5-HT含量显著升高。结论:黑水缬草石油醚部位高、中、低3个剂量均能明显延长小鼠睡眠时间。黑水缬草石油醚部位改善睡眠作用的机制与脑内神经递质GABA,5-HT的含量有关。

Objective： To study the sleep improvement of petroleum extract of Valeriana amurensis, observe it＇s effect on the contents of γ-aminobutynic acid （GABA） and 5-hydroxytryptamine （5-HT） in the brain of mice, and preliminarily investigate the activity mechanism. Method： The effects of three doses of petroleum extract of V. Amurensis, combined with Flumazenil, 5-hydroxytryptophane （5-HTP） and parachlorophenylalanine （PCPA）, respectively on sleep latency duration and sleep period of mice were examed with the synergy of sodium pento-barbital. The improved-sleeping mechanism of petroleum extract was determined by detecting the contents of GABA and 5-HT in the brains of mice with ELISA method. Result： Three doses （30, 15, 7.5 g·kg-1） of V. amurensis petroleum extract showed the effect of improving sleep by shortening the sleep latency and prolonging the sleep duration of mice. Compared with the control. The synergy effect of diazepam or V. amurensis petroleum extract with sodium pentobarbital was blocked by Flumazenil injection. The synergy effect of V. amurensis petroleum extract with sodium pentobarbital was intensified by 5-HTP. The synergy effect of V. amurensis petroleum extract with sodium pentobarbital was blocked by PCPA. The contents of GABA and 5-HT in the brain of mice were increased by the three doses of V. amurensis petroleum extract. Conclusion： V. amurensis petroleum part （30, 15, 7.5 g·kg-1） could significantly prolong the sleep duration of mice. The improved-sleeping mechanism of V. amurensis petroleum extract was verified to exist the relationship with the content of GABA and 5-HT in mouse brain.