摘要
目的:研究当归挥发油对实验性哮喘大鼠的平喘作用及其对肺组织相关转录因子表达的影响。方法:采用注射卵清白蛋白(OVA)与氢氧化铝致敏、OVA雾化吸入激发来复制哮喘动物模型,通过观察OVA造模过程中大鼠行为学的变化、肺组织T-bet与GATA-3蛋白表达以及肺组织病理学改变,研究当归挥发油的平喘作用及其作用机制。结果:当归挥发油可明显减小哮喘大鼠在造模过程中的行为综合评分值,提高肺组织T-bet蛋白的表达而降低GATA-3蛋白的表达水平,改善肺组织病理学,减轻支气管上皮细胞脱落程度与炎性细胞浸润程度。结论:当归挥发油具有一定的平喘作用,而调节T-bet/GATA-3表达失衡是其作用机制之一。
Objective： To investigate the effects of Volatile Oil of Radix Angelicae Sinensis（VOA） on anti-asthma and expression of key transcription factors in the lung in rat experimental asthmatic model. Methods： Ovalbumin（OVA） and Al（OH）3adjuvant mixture was injected followed by OVA inhaling to set up the rat asthmatic model. The anti-asthmatic mechanisms of VOA were evaluated through the changes of rat＇s behaviors,the protein levels of T-bet and GATA-3 in the lung,and the lung histology. Results： VOA could significantly decrease the general scores of rats,enhance the protein levels of T-bet and decrease GATA-3 in the lung,improve the lung function,attenuate shedding epithelial cell of bronchial wall and inflammatory cells in the lung. Conclusion： VOA has some effects of anti-asthma and one of mechanisms is to adjust the equilibrium of T-bet / GATA-3.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第11期1142-1145,1150,共5页
Chinese Journal of Immunology
基金
国家科技支撑计划项目子课题(2011BAI05B02)
甘肃省中药药理与毒理学重点实验室开放基金项目(No.ZDSYS-KJ2012-002)
