IL-6、IL-10、IL-17在慢性乙型病毒性肝炎患者血中水平分析

Analysis on the levels of IL-6,IL-10 and IL-17 in serum of patients with chronic hepatitis B

目的:探讨血清IL-6、IL-10、IL-17在慢性乙型肝炎发生发展中的作用及意义。方法:采用酶联免疫吸附法(ELISA法)检测70例CHB患者和20例健康体检者(对照组)血清中IL-6、IL-10、IL-17水平及其与乙肝患者肝功能指标及血清中病毒载量的关系。结果:与健康对照组相比较,CHB患者IL-6、IL-10、IL-17均明显升高(P<0.05);与HBeAg阴性组比较,HBeAg阳性组IL-6水平下降(P<0.05),而IL-17水平明显升高(P<0.05),IL-10水平无差异;3种细胞因子水平在HBVDNA≥105拷贝/ml和<105拷贝/ml2组间无差异;IL-6与AST、TBIL、HBV-DNA均呈正相关关系。结论:IL-6、IL-10、IL-17均参与CHB的发病过程,其含量的变化与肝脏炎症程度及病毒复制情况密切相关。

Objective： To explore the level of cytokines and their function in serum of patients with hepatitis B. Methods：Blood samples from 70 CHB patients and 20 healthy controls were collected. The levels of IL-6,IL-10 and IL-17 were detected by ELISA. We also tested the liver function of patients. Results： Compared with the healthy controls,CHB patients were significantly higher in the level of IL-6,IL-10 and IL-17（ P 0. 05）; compared with HBeAg negative,HBeAg positive were significantly lower in the level of IL-6（ P 0. 05）,while the level of IL-17 were higher（ P 0. 05）,the level of IL-10 was almost similar. The level of 3 cytokines was similar between HBV-DNA≥105copies / ml and 105copies / ml. IL-6 level showed positive correlation on AST,TBIL and HBV-DNA. Conclusion： IL-6,IL-10 and IL-17 might be involved in the pathogenesis of CHB,which level＇s alteration has a closer relationship with the level of liver inflammation and HBV DNA.