摘要
目的:探讨茯苓多糖对小鼠外周血免疫球蛋白IgA、IgG和IgM合成水平的影响。方法:50只成年昆明种雄性小鼠随机分成5组,生理盐水对照组与盐酸左旋咪唑对照组分别灌注生理盐水与2.5 g/L盐酸左旋咪唑溶液,茯苓多糖低、中、高剂量组分别灌注1、2、4 g/L的茯苓多糖溶液,持续一周后摘眼球取血,采用ELISA法测定各组小鼠外周血清中IgA、IgG和IgM含量。结果:茯苓多糖处理组IgA、IgG和IgM水平高于生理盐水对照组,组间差异均有统计学意义(P<0.01);IgA、IgG和IgM水平与茯苓多糖剂量呈正相关(IgA:r s=0.934,P<0.01;IgG:r s=0.943,P<0.01;IgM:r s=0.939,P<0.01)。结论:茯苓多糖可促进小鼠血清IgA、IgG和IgM的生物合成,且存在剂量-效应关系,作用随茯苓多糖浓度的增大而增强。
Objective: To explore the influence of Pachyman on biosynthesis level of mice peripheral blood immunoglobulins IgA,IgG and IgM. Methods: 50 adult male Kunming mice were randomly divided into five groups,in which the saline and levamisole hydrochloride control group were respectively perfused with saline and 2. 5 g / L levamisole hydrochloride solution,pachyman in low,medium and high dose groups,were respectively perfused with Pachymaran of 1,2 and 4 g / L. A week later,the blood of the eyeball was taken out to measure the concentration of mice peripheral blood immunoglobulins IgA,IgG and IgM by the ELISA method. Results: Pachyman processing groups IgA,IgG and IgM levels were higher than the saline control group,and group differences were statistically significant( P 0. 01); IgA,IgG and IgM levels Pachymaran dose was positively correlated( IgA: r s= 0. 934,P 0. 01;IgG: r s= 0. 943,P 0. 01; IgM: r s= 0. 939,P 0. 01). Conclusion: Pachyman can promote the biosynthesis of mice serum IgA,IgG and IgM,and the dose-response relationship is obvious. The effect can be enhanced with the increase of the Pachyman concentration.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第11期1213-1215,共3页
Chinese Journal of Immunology
基金
湖南省教育厅科研项目(10C00278)