茯苓多糖对小鼠血清IgA、IgG和IgM生物合成水平的影响

Influence of Pachyman on biosynthesis level of mice serum IgA,IgG and IgM

目的:探讨茯苓多糖对小鼠外周血免疫球蛋白IgA、IgG和IgM合成水平的影响。方法:50只成年昆明种雄性小鼠随机分成5组,生理盐水对照组与盐酸左旋咪唑对照组分别灌注生理盐水与2.5 g/L盐酸左旋咪唑溶液,茯苓多糖低、中、高剂量组分别灌注1、2、4 g/L的茯苓多糖溶液,持续一周后摘眼球取血,采用ELISA法测定各组小鼠外周血清中IgA、IgG和IgM含量。结果:茯苓多糖处理组IgA、IgG和IgM水平高于生理盐水对照组,组间差异均有统计学意义(P<0.01);IgA、IgG和IgM水平与茯苓多糖剂量呈正相关(IgA:r s=0.934,P<0.01;IgG:r s=0.943,P<0.01;IgM:r s=0.939,P<0.01)。结论:茯苓多糖可促进小鼠血清IgA、IgG和IgM的生物合成,且存在剂量-效应关系,作用随茯苓多糖浓度的增大而增强。

Objective： To explore the influence of Pachyman on biosynthesis level of mice peripheral blood immunoglobulins IgA,IgG and IgM. Methods： 50 adult male Kunming mice were randomly divided into five groups,in which the saline and levamisole hydrochloride control group were respectively perfused with saline and 2. 5 g / L levamisole hydrochloride solution,pachyman in low,medium and high dose groups,were respectively perfused with Pachymaran of 1,2 and 4 g / L. A week later,the blood of the eyeball was taken out to measure the concentration of mice peripheral blood immunoglobulins IgA,IgG and IgM by the ELISA method. Results： Pachyman processing groups IgA,IgG and IgM levels were higher than the saline control group,and group differences were statistically significant（ P 0. 01）; IgA,IgG and IgM levels Pachymaran dose was positively correlated（ IgA： r s= 0. 934,P 0. 01;IgG： r s= 0. 943,P 0. 01; IgM： r s= 0. 939,P 0. 01）. Conclusion： Pachyman can promote the biosynthesis of mice serum IgA,IgG and IgM,and the dose-response relationship is obvious. The effect can be enhanced with the increase of the Pachyman concentration.