摘要
目的:确定葡萄籽中多酚提取的最佳工艺。方法:以葡萄籽为原料,采用超声波辅助提取葡萄籽多酚。比较酒石酸亚铁法和Folin-Ciocalteau法测定多酚含量的不同,在单因素实验的基础上,应用Box-Behnken实验设计和响应面分析法,探讨液料比、超声时间、超声功率、浸提温度对多酚提取量的影响。结果:超声波辅助提取葡萄籽多酚的最佳工艺条件为液料比13∶1(mL/g)、时间100min、超声功率36W、浸提温度45℃,葡萄籽多酚提取量可达17.22mg/g,提取量较高。结论:这种方法可以避免多酚在较高温度下的分解,且优化后提取率较高,可以用于葡萄籽多酚的提取。
Objective.To establish optimum conditions for extracting polyphenols from grape seeds. Methods= Polyphenols were extracted from grape seeds by ultrasonic-assisted ethanol extraction. Different influence of ferrous tartrate and Folin-Ciocalteau method was compared. On the basis of single factor experiment, response surface methodology based on Box-Behnken experimental design was used to explore the effect of liquid-tosolid ratio,ultrasonic treatment time,power and temperature on extraction efficiency. Results.The optimum extraction conditions were 13:1 (mL/g), 100min,36W and 45℃ for liquid-to-solid ratio,ultrasonic treatment time, ultrasonic power and temperature, respectively. Under these conditions, the extraction content of polyphenol was 17.22mg/g. Extraction rate was high. Couclusions.Such extraction metod could effectively avoid polyphenol decomposition under temperature and yield was higher. The method could be widely applied in polyphenols extraction.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第24期279-283,287,共6页
Science and Technology of Food Industry
基金
吉林省科技发展计划项目(20120919)
关键词
葡萄籽
多酚含量
超声提取
响应面法
grape seeds
polyphenol content ultrasonic-assisted response surface methodology