油茶根系及分泌物中有机酸的HPLC法测定

Determination of organic acids in oil tea root tissue and root exudation with HPLC

以沙培油茶嫁接苗为试材，建立了油茶根系组织和根系分泌物有机酸检测方法，并测定了不同供磷条件下油茶根系组织和分泌物中的有机酸。结果表明：利用溶液培养法收集根系分泌有机酸和利用水浴超声萃取根系组织中的有机酸效果较好；建立的油茶根系组织和根系分泌物10种有机酸高效液相色谱检测方法线性相关系数为0．9990～0．9999，回收率为82．3％～108．4％，相对标准偏差为0．72％-3．46％，检测限为1—193ug／L，符合高效液相色谱测定的要求，适用于油茶根系组织和分泌物中多种有机酸的快速同时测定；应用该方法检测发现，无磷处理下油茶根系组织和分泌物中10中有机酸含量均显著高于有磷处理，说明低磷可促进有机酸在油茶根系组织中的积累和向根外分泌。

A simple and efficient method was developed for the determination of organic acids in Camellia oleifera root tissue, and the samples cultured in sand with different phosphorus concentration were determined. The root exudation was collected by the solution culture method and the organic acids were extracted from the dry root powder with ultrasonic extraction in a water bath. The correlation coefficient, recoveries, coefficient variations and limit of detection of the meth- od was 0. 999 0 -0. 999 9, 82. 3% - 108.4%, 0. 72% -3.46%, 1 - 193 ug,/L, respectively, suggesting the 10 organ- ic acids could be determined simultaneously and quickly by the method. The 10 organic acids of the samples determined by the method. The results showed that the content of organic acids in root tissue and root exudation under no phosphorus stress were higher than that under phosphorus stress, it means that the phosphorus stress encourage the organic acids accu- mulate in camellia＇s roots and secret intn rhizoanhere_