黄瓜细菌性萎蔫病菌分子诊断研究

Study on molecular diagnosis of cucumber bacterial wilt

从田间黄瓜细菌性萎蔫病植株分离获得病原菌,比对其近源种的16S rDNA序列,确定该病原菌为嗜维管束欧文氏菌(Erwinia tracheiphila),并设计出特异引物ET-P1/ET-P2.经过对该引物的PCR扩增条件优化,扩增出一条794 bp的黄瓜细菌性萎蔫病菌特异条带,检测灵敏度达100 fg/μL,可准确扩增出黄瓜发病植株和根围土壤中的DNA片段.

The pathogen was obtained by separating the cucumber bacterial wilt plant,and by comparing with the closely-related species 16Sr DNA sequence,it was identified as Erwinia tracheiphila and specific primers ET-P1 / ET-P2 was designed. A 794 bp cucumber bacterial wilt special band can be amplified by optimizing the PCR amplification of ET-P1 / ET-P2 primers with the detection sensitivity of 100 fg / μL,and can amplify the DNA of bacterial wilt cucumber and rhizosphere soil. The molecular diagnosis technology of cucumber bacterial wilt.