烤后烟叶SSR反应体系的优化研究

The optimization research of flue-cured tobacco SSR reaction system

以烤烟RG11品种为原料,研究烤后烟叶简单重复序列(SSR)分析中聚合酶链式反应(PCR)体系各主要因素及其之间的相互作用对SSR扩增的影响,结果表明:烤后烟叶SSR-PCR反应最佳体系为20μL体系中含35 ng模板DNA,1.5 U TaqDNA聚合酶,2.375 mmol/L MgCl2,0.6 mmol/L dNTPs和0.4μmol/L引物.

Using flue-cured tobacco varieties RG11 as raw materials,the influence of simple sequence repeat（ SSR） amplification on flue-cured tobacco SSR analysis of polymerase chain reaction（ PCR） system of the main ingredients and among was studied,and the results showed that flue-cured tobacco SSR-PCR for20 μL system,including template DNA 35 ng,1. 5 U TaqDNA polymerase,2. 375 mmol / L MgCl2,0. 6 mmol / L dNTPs,0. 4 μmol / L primer.