敦煌葆元膏对下丘脑-垂体-肾上腺轴作用的实验研究

Experimental Study on the Effects of DunHuang BaoYuan Ointment on Hypothalamic-pituitary-adrenal Axis

目的:探讨敦煌葆元膏对慢性疲劳综合征模型大鼠下丘脑-垂体-肾上腺轴的影响,以了解敦煌葆元膏补肾功能的生理基础点。方法:将40只慢性疲劳模型大鼠随机分为高(4.2 g/kg)、中(1.4 g/kg)、低(0.7 g/kg)剂量组及模型对照组各10只,另设空白对照组10只,灌胃1周后大鼠股动脉采血检测血清中促肾上腺皮质激素释放激素(CRH)、五羟色胺(5-HT)以及皮质酮(Cort.)的含量。结果:敦煌葆元膏高、中、低剂量组血清CRH、5-HT以及Cort.含量较高,模型对照组与空白对照组含量较低。结论:敦煌葆元膏可能通过调节HPA中相关激素含量水平,激发其生理功能,从而发挥补肾的药理作用。

Objective： To explore the effects of DunHuang BaoYuan ointment on hypothalamic-pituitary- adrenal axis of the rats with chronic fatigue syndrome and know physiological basic points for kidney-nourishing of DunHuang BooYuan ointment. Method： Forty rats were randomized into four groups： high （4.2 g/kg） dosage group, moderate （1.4g/kg） dosage group, low （0.7g/kg） dosage group and model control group. Another ten rats were established as blank control group. After lavage for one week, the contents ofcorticosterone（Cort.）, serotonin （5-HT） and corticotrophin releasing hormone （CRH） were detected in the serum of femoral blood. Result： The contents of CRH, 5-HT and Cort. were higher in the groups of high dosage, moderate dosage and low dosage. The contents of the indexes in model control group and blank control group were lower. Conclusion： DunHuang BaoYuan ointment could play the function of kidney-nourishing by regulating associated hormone level in PHA that motivates its physiological function.