酒精度对高效液相色谱-荧光法分析白酒中氨基甲酸乙酯含量的影响

Effect of alcoholic strength on the determination of ethyl carbamate in Chinese spirits byhighperformance liquid chromatography-fluorescence detection

分析酒精度对高效液相色谱-荧光（HPLC-FLD）法测定白酒中氨基甲酸乙酯（EC）含量的影响,建立了快速检测白酒中EC含量的HPLC-FLD分析方法。通过比较不同酒精度下同一EC含量的峰面积,研究酒精度对EC含量测定的影响。结果表明,在5%~65%（v/v）酒精度范围内,酒精度与EC含量（峰面积）具有良好的线性关系,相关系数R2大于0.98,同时建立了不同酒精度间EC含量换算的相对校正系数。该方法在10~500μg/L范围内呈良好的线性关系,R2大于0.999 9,定量限为10μg/L,加标回收率为98.9%~108.2%,相对标准偏差为0.6%~4.9%。评价了HPLC-FLD法的可靠性,将该方法与GC-MS方法比较,结果显示两种方法无明显差异。该方法简单、灵敏、准确,适用于白酒中EC含量的快速测定,具有较好的实际应用价值。

A high performance liquid chromatography-fluorescence detection （HPLC-FLD） method was established for rapid determination of ethyl carbamate （EC） in Chinese spirits. Through the analysis and comparison of the EC peak areas in different alcoholic strengths deter- mined by HPLC-FLD, the effect of alcoholic strength on the determination of the content of EC was found. The alcoholic strength and the peak area of EC showed good linearity in the range of 5% to 65% （v/v） alcohol content, and the correlation coefficients （R2） were higher than 0.98. Furthermore, the conversion between the peak area of EC with different alcoholic strengths was established by the relative correction coefficient：. The method showed a good lin- earity in the range of 10 to 500 ~g/L for EC with the average recoveries of 98.9% - 108.2%and RSDs of 0.6% - 4.9%. The reliability of the established HPLC-FLD method was evaluated by comparison with GC-MS method. The results showed that the results of two methods were not significantly different, The developed method is simple, sensitive, accurate, and suitable for the rapid determination of EC in Chinese spirits.