大肠肿瘤石蜡标本P53 mRNA RT-PCRIS比较研究

Comparative study on P53 mRNA in colorectal tumor paraffin embedded samples by RT PCRIS

目的 :探讨大肠肿瘤P5 3mRNA转录改变。方法 :对 9例大肠癌和 3例良性大肠肿瘤石蜡标本的肿瘤灶、移行区和自身正常肠组织 ,应用反转录原位PCR技术进行了研究。结果 :3例良性肿瘤中仅 1例在肿瘤区组织检出弱阳性。 9例大肠癌中 ,肿瘤区检出 7例阳性 ,移行区检出 4例阳性 ,自身正常肠组织检出 1例阳性。结论 :在肿瘤早期即可能出现P5 3基因mRNA改变 ,应用RT CPRIS检出p5 3mRNA的阳性率高于常规免疫组化法。RT PCRIS具有很高的灵敏度和特异性 。

Purpose:To probe the variation of p53 mRNA transcriptions in of colorectal neoplasia.Methods:Tumor tissues, transitonal tissues and normal tissues of 9 cases of colorectal carcinomas and 3 case benign neoplasia were comparatively studied by reverse transcriptional polymerase chain reaction in stiu (RT PCRIS). Results:In 3 benign cases, only 1 positive was found in the tumor. In 9 malignant cases,the positives cases were: 7 in neoplastic tissues; 4 in transitional tissues; 1 in normal tissue.Conclusions:The results show that the variation of P53 mRNA transcription may occur in the early phase of colorectal neoplasia development, and that the positive rate of P53 mRNA by RT PCRIS is higher than the conventional immunohistochemistry (IHC). RT PCRIS analysis combined with organ conformation has very high accuracy and specificity