胃癌患者手术前后NK及T细胞的改变

Changes of preoperative and postoperative NK and T lymphocyte with gastric cancer

目的 :评价胃癌患者手术前、后NK及T细胞动态变化。方法 :采用单克隆抗体方法及MTT法检测了36例胃癌患者手术前、后T淋巴细胞亚群 (TC)及自然杀伤细胞 (NK)活性 ,并与 30例正常对照进行了比较。结果 :术前患者CD3(+)、CD4(+)、CD4(+) /CD8(+)比值、NK细胞活性显著降低 (P <0 0 1。CD8(+)细胞增高 (P <0 0 1)。术后 2 0天 ,患者细胞免疫功能逐渐恢复。与术前比较除CD4(+)外 ,其余均有显著性意义 (P <0 0 5 )。结论 :胃癌患者细胞免疫功能与肿瘤的消长有密切关系。

Purpose:The aim of this study was to evaluate the changes of NK and T lymphocyte with gastric cancer and before and after operation.Methods:T lympho cyte subsets and NK cell activity of 36 cases of gastric cancer and 30 cases of normal subjects were measured by monoclonal anti human T lymphocyte antibody and MTT assay before and after operation.Results:It was found that before operation the CD3(+),CD4(+) cell and ratio of CD4(+)/CD8(+) were significantly decreased and CD8(+) cell was increased and NK activity decreased with gastric cancer as compared to control group ( P <0 01). But cell immune function gradually recovered 20 days later. all were significant before and after operation except for CD4(+) ( P <0 05). Conclusions:Cell immune function in patients with cancer were related to tumor progress and T lymphocyte subsets and NK cell activity could be used as important monitoring indicators.