正常SD大鼠组织钠泵α亚单位的基因表达

Distribution of sodium pump α subunit isoforms in normal sprague dawley rats

目的 系统研究正常SD大鼠各组织内钠泵α亚单位三种异构体的分布情况。方法 采用分子生物学RT PCR及免疫组织化学技术 ,检测正常大鼠多种组织内钠泵α1、α2及α3亚单位mRNA及蛋白水平的表达。结果 左室心肌主要表达α1与α2亚单位 ,α3亚单位表达较弱 ;肝脏中在mRNA水平α1亚单位表达较强 ,α2与α3亚单位较弱 ,而在蛋白水平三者表达均较弱 ;肾脏中主要表达α1亚单位 ,α2与α3亚单位较弱 ;肾上腺中α1亚单位的表达略高于α2与α3亚单位 ;动脉平滑肌中α2亚单位的表达略高于α1与α3亚单位 ;垂体中三种亚单位的表达均较强 ;下丘脑中α2与α3亚单位表达较强 ,α1亚单位表达较弱。结论 本研究系统揭示了正常大鼠多种组织内钠泵α亚单位三种异构体在mRNA及蛋白水平的表达情况 。

Objective To explore the distribution of sodium pump α subunit isoforms at both mRNA and protein level in normal Sprague Dawley(SD) rats.Methods With RT PCR(reverse transcription polymerase chain reaction) and immunohistochemical assay,sodium pump α1 ,α2 ,and α3 subunit isoforms were detected at mRNA level and protein level in the myocardium,liver,kidney,adrenal gland,aortic smooth muscle,pituitary and hypothalamus in normal rats.Results Sodium pump α1 ,α2 , and α3 subunit isoforms distribute in a tissue specific fashion in normal SD rats.α1 and α2 are the main isoforms and α3 is much less in myocardium.α1 is more than α2 or α3 isoform at mRNA level in liver,while all of α1 ,α2 , and α3 isoform are less expressed at protein level.α1 isoform is abundantly but α2 and α3 are less expressed both at mRNA and protein levels in kidney.α2 isoform is just more than α1 and α3 isoforms in aortic smooth muscle.All of three isoforms are expressed abundantly in pituitary.α2 and α3 are more and α1 is less expressed in hypothalamus. Conclusion The results of this study have revealed the distribution of sodium pump α subunit isoforms at both mRNA and protein level in normal SD rats,which might be helpful for the further studying the physiologic and pathologic roles of sodium pump.