聚合酶链反应早期诊断结核性关节炎的临床价值

The clinical value of PCR for tuberculosis arthritis early diagnosis

目的 探讨聚合酶链反应 (PCR技术 )对诊断结核性关节炎的临床价值。方法 对 42例结核性关节炎与 2 0例非结核性关节炎的关节积液分别应用抗酸染色镜检 ,分离培养和PCR检测。结果 42例结核性关节积液三种方法的检出率分别为 :镜检法 1 9 0 % ,培养法 38 1 % ,PCR法 66 7% ,后者显著高于前二者 (P <0 .0 1 )。 2 0例非结核性关节积液抗酸染色镜检和分离培养结果均为阴性 ,PCR阳性率 1 0 %。结论 PCR技术检测关节积液中的结核杆菌具有快速、敏感和高效等优点 。

Objective In order to evaluate the clinical value of polymerase chain reaction(PCR) for tuberculosis arthritis early diagnosis.Methods The arthritis fluid of cases was analyzed respectively.It was inspected by the method of anti acid dyeing microscope observing,culturing and PCR inspecting parately.The 42 cases was suffered from tuberculosis arthritis and 20 cases from the other arthritis.Results The assay of tubercle bacillus was 19.10% by microscope,38.1% by culture and 66.7% by PCR.The last assay was more outstanding than others( P <0.01).To the 20 cases suffered from un tuberculosis arthritis,the assay was negative by microscope and culture,it was 10% by PCR.Conclusion PCR method is rapid sensitive and efficientive in inspecting tubercle bacillus from arthritis fluid.It is very valuable for tuberculosis arthritis early diagnosis.