五味子水提物对微波辐射引起大鼠肝细胞氧化应激损伤的预防作用

Preventive effect of schisandrae water extract on oxidative stress injury of liver cells of rats induced by microwave radiation

目的:探讨五味子水提取物预防给药对微波辐射引起的大鼠肝细胞氧化应激损伤的影响,阐明五味子水提取物对微波产生的肝损伤的预防和保护作用。方法:36只正常成年Wistar大鼠随机分为正常对照组、微波辐射组和五味子水提取物预防给药组,每组12只。五味子水提取物预防给药组大鼠给予五味子水提取物灌胃,5d后与微波辐射组均进行微波辐射,并在微波辐射后0和16h分别检测各组大鼠肝组织丙二醛(MDA)浓度和SOD活性,HE染色观察各组大鼠肝组织和细胞形态学。结果:与正常对照组比较,辐射后0h微波辐射组大鼠肝组织MDA浓度和SOD活性均无明显变化;而辐射后16h微波辐射组大鼠肝组织MDA明显增加,SOD活性明显降低(P<0.05);微波辐射16h后,与微波辐射组比较,五味子水提取物预防给药组大鼠MDA浓度减少,且SOD活性增大(P<0.05)。微波辐射引起大鼠肝细胞明显水肿,而五味子水提取物预防给药可明显缓解微波辐射引起的大鼠肝细胞水肿。结论:200mW·cm-2的微波辐射可引起大鼠肝组织氧化应激水平和肝细胞形态明显改变,而五味子水提取物对微波辐射引起的Wistar大鼠肝细胞损伤有明显的预防作用。

Objective To study the influence of schisandrae water extract in the oxidative stress injury of liver cells of the rats induced by microwave radiation, and to clarify the preventive effect of schisandrae water extract on hepatic injury induced by microwave radiation in rats. Methods 36 normal adult Wistar rats were randomly divided into normal control group （n= 12）, microwave radiation group （n=12）, and schisandrae water extract prevention group （n = 12）. The rats in schisandrae water extract prevention group were pretreated individually with schisandrae water extract for 5 d before microwave radiation, after microwave radiation and schisandrae water extract treatment, the concentration of MDA and the activity of SOD of the rats 0 and 16 h after radation in various groups were detected, and the morphological changes of the liver tissue and liver cells were observed by HE staining. Results The concentration of MDA and the activity of SOD of the rats in microwave radiation group had no significant difference compared with normal control group 0 h after microwave radiation. 16 h after microwave radiation, the concentration of MDA was increased （P〈0.05）, and the activity of SOD of the rats in microwave radiation group was obviously decreased （P〈0.05）. The concentration of MDA was decreased and the activity of SOD was increased in schisandrae water extract prevention group （P〈0.05）. Microwave radiation casused obvious edema of the liver cells, but the pathological injury of the liver tissue in the rats in schisandrae water extract prevention group was improved. Conclusion 200 mW·cm^-2 microwave radiation can induce the changes of oxidative stress level and morphology of liver cells of the rats, and schisandrae water extract shows obvious therapeutical effects on the stress iniury of liver cells in Wistar rats induced by microwave radiation.