池沼公鱼肌肉中脂肪酸成分的GC-MS分析

Fatty Acids Analysis in Hypomesus olidus Muscle by GC-MS

目的:分析测定池沼公鱼肌肉中的脂肪酸成分.方法:采用索氏提取法提取池沼公鱼肌肉中的脂溶性成分,经甲酯化处理后用气相色谱-质谱联用技术(GC-MS)分离和鉴定,运用面积归一化法确定各个成分的相对百分含量.结果:从池沼公鱼脂肪油中鉴定出38种脂肪酸,其中不饱和脂肪酸21种,主要种类有:棕榈油酸(8.11%),(E)-9-十八碳烯酸(12.25%),顺式二十碳五烯酸(8.43%),二十二碳六烯酸甲酯(11.85%)等;饱和脂肪酸17种,主要种类有:棕榈酸(8.13%),肉豆蔻酸(2.71%),十八烷酸(4.23%)等.结论:试验结果表明,池沼公鱼的营养价值较高,具有很好的开发利用价值.

Objective ：The research aimed to determine and analyze the fatty acid compositions of Hypomesus olidus muscle. Method： The lip soluble components were extracted from Hypomesus olidus muscle by Soxhlet extraction respectively. The extracted fatty acids were identified by gas chromatography-mass spectrometry （GC-MS） after methyl etherification treatment, and the relative percentage of each component was confirmed by peak-area normalization method. Result： Total 38 kinds of fatty acid, including 21 kinds of unsaturated fatty acid and 17 kinds of saturated fatty acid were identified from oil of Parthenoeissus quinquefolia muscle. The main components of unsaturated fatty acids were palmitoleic acid （8.11% ） ,9-octadecenoic acid, （ 12.25% ）, all z,5,8,11,14,17-eicosapentaenoic acid （ 8.43% ） ,4,7,10,13,16,19-docosahexaenoic acid （ 11.85% ）, etc. While the main components of saturated fatty acids were palmitic acid （ 8. 13% ）, tetradecanoic acid （ 2.71% ）, octadecanoic acid （4.23 % ）, etc. Conclusion： This result provides the nutrition and health value of Hypomesus olidus were higher and it had better-development and utilization prospect.