血清SOD、TNF-α及荧光实时定量PCR检测在婴幼儿支原体肺炎中的应用

Applications of serum SOD detection,TNF-α detection,and florescent real-time quantitative RT-PCR in diagnosis and treatment of infants with mycoplasma pneumoniae pneumonia

目的：探讨血清中超氧化物歧酶（SOD）、肿瘤坏死因子（TNF-α）及荧光实时定量PCR（FQ—PCR）检测在婴幼儿支原体肺炎（MPP）早期诊断和治疗中的价值。方法：选择2010年1月-2011年12月肺炎患儿136例，x光检查肺部有明显改变、血清MP抗体4倍及以上滴度升高为MPP患儿，余下为非MPP患儿。分别检测患儿治疗前后的血清SOD、TNF—α浓度及对部分患儿取肺泡灌洗液后进行FQ—PCR；以80份健康儿童血清作为阴性对照。组间率的比较采用χ2检验，计量资料采用t检验，双变量相关分析采用Pearson检验。结果：治疗前MPP患儿血清中SOD和TNF—α浓度均显著高于对照组（P〈0．01）。相关分析显示，MP患儿组的血清SOD、TNF-α水平与PCR检测结果呈正相关，对照组的血清水平与PCR检测结果无相关性；治疗后MPP患儿组的SOD和TNF-α浓度水平均显著下降（P〈0．01）。结论：联合测定血清中SOD、TNF—α水平及FQ—PCR对MPP患儿的早期诊断、治疗和预后有十分重要的临床价值，可作为评价MPP的转归以及判断临床疗效的指标。

Objective： To explore the values of serum superoxide dismutase enzyme （SOD） detection, tumor necrosis factor -α （ TNF - α） detection, and florescent real - time quantitative RT - PCR （ FQ - PCR） in early diagnosis and treatment of infants with myco- plasma pneumoniae pneumonia （MPP） . Methods： A total of 136 infants with pneumonia were selected from January 2010 to December 2011, the infants with obvious lung changes displayed by A radiology and serum MP antibody titer increasing by four times or more than four times were diagnosed as MPP group, and the other infants were designed as non - MPP group ; the concentrations of serum SOD and TNF - α before and after treatment in the two groups were detected respectively ; FQ - PCR was conducted in bronchoalveolar lavage fluid specimens of some infants; 80 healthy infants were selected as control group; χ2 test was used to compare the rates among different groups, t test was used to compare measurement data, bivariate correlation was conducted with Pearson test. Results： The serum concentrations of SOD and TNF - α in MPP group before treatment were statistically significantly higher than those in control group （P 〈 0. 01 ） ; the results of correla- tion analysis showed that there was a positive correlation between serum SOD and TNF -α levels and PCR results in MPP group, while in control group, there was no correlation between serum SOD and TNF - α levels and PCR results ; after treatment, the concentrations of serum SOD and TNF - α in MPP group decreased significantly （P 〈0. 01 ） . Conclusion： Joint detection of serum SOD, TNF - α and FQ - PCR has very important clinical value in early diagnosis, treatment and prognosis of MPP in children, which can be used as an index to evaluate prognosis of children with MPP and predict the clinical efficacy.