精液白细胞相关因子含量变化与男性不育的相关性研究

Study on the correlation between change of leukocyte-related factors in sperm and male sterility

目的:探讨精液白细胞相关因子含量变化与男性不育的相关性。方法:该实验收集318例男性不育患者精液标本,采用过氧化物酶染色法检测精液白细胞,采用WHO第5版标准分析精子密度及活力等精液参数,采用ELISA及透射比浊法分别测定精液肿瘤坏死因子-α(TNF-α)、白介素-2(IL-2)和C-反应蛋白(CRP)含量。结果:318例男性不育患者中白细胞组占132例(41.51%);非白细胞组占186例(58.49%);白细胞组的精子密度、精子活动率、精子活力及精子顶体酶活性均低于非白细胞组,差异有统计学意义(P<0.05);而白细胞组的TNF-α、IL-2、CRP、精浆抗体(AsAb)及支原体阳性率均高于非白细胞组,差异有统计学意义(P<0.05)。结论:精液中白细胞、TNF-α、IL-2及CRP含量变化可反映生殖系统免疫状态,与男性不育密切相关。

Objective： To explore the correlation between change of leukocyte -related factors in sperm and male sterility. Methods： The sperm specimens of 318 sterile men were collected, then sperm leukocytes were detected by peroxidase staining, the sperm parameters （density, activity） were analyzed according to the fifth edition of the WHO criteria; ELISA and transmission turbidimetry were used to detect the contents of tumor necrosis factor - α （ TNF - α ）, interleukin - 2 （ IL - 2 ）, and C - reactive protein （CRP） . Results： The ster- ile men were divided into leukocyte group （ 132 patients, 41.51% ） and non - leukocyte group （ 186 patients, 58.49% ） ; sperm density, sperm activate rate, sperm activity, and acrosin activity in leukocyte group were statistically significantly lower than those in non -leukocyte group （P 〈0. 05）; but the contents of TNF- α, IL- 2, CRP, antisperm antibody （AsAb）, and positive rate of mycoplasma in leukocyte group were statistically significantly higher than those in non - leukocyte group （ P 〈 0. 05） . Conclusion： The changes of leukoeytes, TNF - α, IL - 2, and CRP in sperm can reflect immune status of reproductive system, which is closely correlated with male sterility.