人类卵裂期胚胎玻璃化冷冻保存技术的临床应用

Clinical application of human embryo vitrification cryopreservation technology during cleavage stage

目的：探讨人类卵裂期胚胎玻璃化冷冻保存技术临床应用的效果。方法：回顾性分析2011年1月～11月在该院生殖中心行玻璃化冷冻解冻患者共43个周期的妊娠情况。结果：玻璃化冷冻解冻共109枚胚胎，全部卵裂球存活87枚（79．82％），存活率94．50％（103／109）；共冷冻解冻43个周期，均有存活胚胎可移植，妊娠率41．86％（18／43），种植率33．01％（34／103）。结论：人类卵裂期胚胎玻璃化冷冻保存可取得较好的结局。

Objective： To explore the clinical application effect of human embryo vitrification cryopreservation technology during cleavage stage. Methods： The pregnancy of patients （43 cycles） undergoing human embryo vitrification frozen -thawed in reproductive cen- ter of the hospital from January to November in 2011 were analyzed retrospectively. Results： A total of 109 frozen - thawed embryos were obtained, 87 blastomeres （79.82%） were alive among all the blastomeres, the survival rate was 94. 50% （103/109） ; there were 43 frozen -thawed cycles, and each cycle had alive embryo for transplantation, the pregnancy rate was 41.86% （18/43）, and the transplantation rate was 33.01% （34/103） . Conclusion： The outcome of human embryo vitrification cryopreservation is good.