p63基因在宫颈癌发展中拷贝数与表达的研究

Study on the copy number and expression of p63 gene in development of cervical cancer

目的:研究p63基因拷贝数与表达的改变与宫颈鳞状细胞癌(SCC)的发生、发展是否存在关联。方法:采用荧光原位杂交(FSIH)和免疫组织化学(IHC)技术分别检测48例宫颈上皮内瘤变(CIN)(CINⅠ级15例、CINⅡ级14例、CINⅢ级19例)及48例SCC组织标本中的p63基因拷贝数及表达变化情况。结果:CINⅠ、CINⅡ、CINⅢ和SCC中的p63基因拷贝数分别为(1.93±0.29)个、(2.52±0.37)个、(3.15±0.52)个和(5.54±0.59)个,CIN组与SCC组比较,差异有统计学意义(P<0.05);随着宫颈病变程度的加深,p63基因阳性表达率逐渐升高,CIN组与SCC组比较,差异有统计学意义(P<0.05)。结论:p63基因拷贝数与表达的增加与宫颈癌的发生、发展密切相关。

Objective： To explore whether there is correlation between changes of copy number and expression of p63 gene and oc- currence, development of cervical squamous cell carcinoma （SCC） . Methods： Fluorescence in situ hybridization （FSIH） and immunohis- tochemical method were used to detect the changes of copy number and expression of p63 gene in 48 cervical intraepithelial neoplasia （CIN） specimens （ 15 specimens of CIN Ⅰ , 14 specimens of CIN Ⅱ , 19 specimens of CIN Ⅲ ） and 48 cervical SCC specimens. Results： The copy numbers of 1363 gene in CIN Ⅰ group, CIN Ⅱ group, CIN Ⅲ group, and SCC group were （1.93 ±0. 29）, （2. 52 ±0. 37）, （3.15 ± 0. 52）, and （5.54 ±0. 59）, respectively, there was statistically significant difference between CIN group and SCC group （P 〈0. 05） ; the positive expression rate of 663 gene increased gradually with the aggravation of cervical lesions, there was statistically significant difference between CIN group and SCC group （ P 〈 0.05 ） . Conclusion： The increases of copy number and expression of io63 gene are closely correla- ted with occurrence and development of cervical SCC.