UPLC法测定藿香正气口服液中3种核苷

Determination of three nucleosides in Huoxiang Zhengqi Oral Liquid by UPLC

目的建立以UPLC法测定藿香正气口服液（苍术、陈皮、厚朴、生半夏等）中尿苷、鸟苷和腺苷量的方法。方法色谱柱为ACQUITYUPLCBEHC，。（1．7}xm，2．1mm×50mm），流动相为水一甲醇梯度洗脱，柱温30℃，体积流量0．5mL／min，检测波长为254nm。结果尿苷在10．29～102．9μ/mL范围内线性关系良好，回归方程为Y=87703X一3811（r=0．9999），平均回收率为97．25％，RSD=2．46％，鸟苷在5．73～57．3μg／mL范围内线性关系良好，回归方程为Y=60604X一1478（r=0．9999）；平均回收率为98．34％，RSD=2．18％，腺苷在5．03～50．3μ斗g／mL范围内线性关系良好，回归方程为Y=61913X一524（r=0．9999），平均回收率为98．88％，RSD：1．89％。结论该方法准确、快速、可靠，能有效地测定藿香正气口服液中3种核苷的量。

AIM To establish an UPLC method for detecting contents of uridine, guanosine and adenosine in Huoxiang Zhengqi Oral Liquid （Atractylodis Rhizome, Citri reticulatae pericarpium , Magnoliae officinalis Cortex, Pinelliae Rhizoma, etc）. METHODS ACQUITY UPLC BEH Cs （1.7 Ixm, 2. 1 mm x 50 mm） was used, and the mobile phase was methanol and water in gradient elution mode, column temperature was 30 C, at a flow rate of 0. 5 mL/min and detection wavelength of 254 nm. RESULTS The linear range was 10. 29 102. 9 ixg/mL for uridin, the regression equation was Y = 87 703X - 3 811 （ r = 0. 999 9 ）. The linear range of guanosine was 5.73 - 57.3 Ixg/mL, the regression equation was Y = 60 604X - 1 478 （ r = 0. 999 9）. The linear range of adeno- sine was 5.03 -50. 3 Ixg/mL, the regression equation was Y=61 913X-524 （r =0. 999 9）. The average recov- eries were 97.25% （RSD = 2. 46% ）, 98.34% （RSD = 2. 18% ） and 98.88% （RSD = 1.89% ）, respectively. CONCLUSION The method is accurate, rapid and reliable and can be used to determine the nucleosides in Huoxiang Zhengqi Oral Liquid.