双酚A抗孕激素样作用的体外观察

In-vitro study of anti progestin like effect of Bishpheonl A

目的观察双酚A在人类子宫内膜Ishikawa细胞中的抗孕激素样作用。方法不同浓度孕酮、双酚A/米非司酮+孕酮混合分别干预细胞48h,RT-qPCR法检测各干预组细胞中PRmRNA表达的情况,且建立S函数量效曲线。Western Blotting检测各组PR蛋白分泌的情况。结果孕酮单独干预下PRmRNA呈下调性表达,孕酮下调PRmRNA的表达受到双酚A、米非司酮浓度依赖性的拮抗,相同现象在蛋白水平上得到确认。结论双酚A能产生与米非司酮近似的抗孕激素样作用。

Objective To observe the anti progestin like effect of Bisphenol A（BPA） in human endometrial Ishikawa cells. Conclusion BPA exerts similar anti-progestin like effects as the well characterized antifertility drug RU486. Methods Ishikawa cell was incubated with progesterone alone and with combinations of progesterone and BPA,Mifepriston（ RU486 ） in different density for 48 h. The PRmRNA expression was tested by RT-qPCR. Sigmoidal Dose-response curves was made accord- ing to the results of RT-qPCR. The protein secretion of PR was tested by Western Blotting. Results PR-mRNA expression was down regulated by progesterone and the down regulating effect of progesterone on PR-mRNA was concentration dependently an- tagonized by RU486. These effects were also confirmed on protein level. Conclusion BPA exerts similar anti progestin like effects as the well characterized antifertility drug RU486.