不同前处理-原子吸收光谱法测定葡萄酒中的微量元素

Determination of Microelements in Wine by Atomic Absorption Spectrometry with Different Pretreatment

[目的]确定利用原子吸收光谱法测定葡萄酒微量元素时的最佳前处理方法。[方法]选用市售澄清干红葡萄酒为样品,对原子吸收光谱法测定葡萄酒中微量元素(铁、锌、镁、钾)时的不同前处理方法进行了比对研究,即直接稀释法、蒸发酒精法、高氯酸-硝酸消化法、双氧水-硝酸消化法,通过方法的精密度、加标回收率及样品测定值等指标对比,确定最佳的前处理方法。[结果]试验表明,双氧水-硝酸消化法测定葡萄酒中微量元素具有更好的效果,是测定葡萄酒中微量元素的理想方法;酒精在葡萄酒的微量元素测定中并无太大影响;葡萄酒微量元素存在结合态,即使是含杂质较少的葡萄酒也应进行消化处理,以保证检测结果的准确性。[结论]研究可为葡萄酒中微量元素的准确检测提供参考依据。

[Objective] To obtain the optimum pretreatment for determination of microelements in wine by atomic absorption spectrometry.[Method] With marketed clarified Ganhong wine as samples,different pretreatment proposal methods for determination of microelements in wine （Fe,Zn,Mg,K） by atomic absorption spectrometry were compared,namely direct dilution method,evaporation of alcohol method,perchloric acid and nitric acid digestion method,hydrogen peroxide and nitric acid digestion method.Through comparison of precision,recovery of standard addition and sample determination value,the optimum pretreatment method was obtained.[Result] The results showed that hydrogen peroxide-nitration method has better results,it is the ideal way for determination of microelements in wine; alcohol has no significant effects on the determination of microelements in wine; Microelements in wine exists combination state,digestion proposal should also conducted on wine with fewer impurities,so as to achieve the accurate detection result.[Conclusion] The study can provide reference for accurate determination of microelements in wine.