外源性硫化氢对过氧化氢灌注损伤大鼠离体心脏的保护作用

Protection of exogenous hydrogen sulfide against isolated rat heart injury perfused with hydrogen peroxide

目的探讨外源性硫化氢对氧化应激损伤大鼠离体心脏的保护作用。方法 SD大鼠离体心脏K-H液平衡灌注20 min后,模型组灌注含有H2O2200μmo·lL-1的K-H液10 min,K-H液60 min;H2O2+NaHS组依次灌注含有H2O2200μmo·lL-1的K-H液10 min,NaHS 10μmo·lL-1的K-H液10 min,K-H液60 min。记录平衡末、处理后10,30和60 min的心率(HR)、左心室收缩压(LVSP)、左心室舒张末压(LVEDP)。灌注结束后检测冠脉漏出液中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量,HE染色法观察心肌组织学变化。结果平衡灌注末各组间心功能指标无统计学差异。与模型组相比,H2O2+NaHS组能显著改善心功能各项指标(P<0.05);冠脉漏出液中LDH〔(884±51)vs(1290±71)U·L-1〕和MDA〔(7.6±0.6)vs(9.3±0.6)nmol·L-1〕的含量下降,SOD含量增高〔(90±11)vs(69±8)U·L-1〕(P<0.05);HE染色可见心肌组织损伤明显减轻。结论外源性硫化氢能减轻大鼠离体心脏氧化应激损伤。

OBJECTIVE To investigate the protective effect of exogenous hydrogen sulfide on the hydrogen peroxide（H2O2）-induced injury in isolated rat hearts. METHODS After a 20 min perfusion with K-H solution in Langendorff perfusion system, hearts in model group were subjected to K-H solution containing H2O2 200 μmol·L-1 for 10 min,hearts in H2O2＋NaHS group,received 10 min of H2O2 200 μmol·L-1 containing buffer followed by another 10 min of NaHS 10 μmol·L-1. Treated with different materials, all hearts were exposed to 60 min repufusion with K-H solution. The heart rate （HR）, left ventricular systolic pressure （LVSP） and left ventricular end diastolic pressure （LVEDP） were recorded.The activity of lactate dehydrogenase （LDH）, malondialdehyde（MDA） and superoxide dismutase （SOD） in coronary effluent fluid was determined. After reperfusion,the rat hearts were removed for HE staining to detect the histomorphological changes in myocardial tissues. RESULTS No differences in baseline hemodynamics were observed among the experimental groups.Compared with model group, H2O2＋NaHS group showed significantly better recovery of hemodynamic function.These improvements were paralleled with the reduction of the activity of LDH（（884±51） vs （1290±71）U·L-1） and MDA（（7.58±0.59） vs （9.3±0.6）nmol·L-1） in the coronary effluent fluid, and the activity of SOD increased（（90±11） vs （69±8）U·L-1）（P〈0.05）, the myocardial damage was attenuated. CONCLUSION Exogenous hydrogen sulfide has protective effect on the H2O2-induced injury in isolated rat hearts.