基于时序表达谱芯片挖掘鸡脂肪酸代谢关键调控基因

Mining Key Genes of Chicken Fatty Acid Metabolism Based on Time Series Microarray

不同脂肪酸的代谢产物决定了鸡肉风味的差异,找出影响鸡肌肉组织中脂肪酸代谢的关键调控基因对提高肉品质以及对优质肉鸡新品系选育具有重要意义。本研究对KEGG和Wikipathway数据库中脂肪酸代谢网络进行整合分析,挖掘鸡脂肪酸代谢的关键基因,通过Gene Spring筛选出不同周龄的肌肉组织中表达谱芯片与关键基因共表达的差异基因(Pearson相关系数≥0.9),然后导入Cytoscape进行共表达网络分析,最后进行跨物种检索关键调控基因功能,得到鸡肌肉组织中脂肪酸代谢的节点基因和相关通路。结果表明,Ep300、Traf2和Pak1等基因是影响鸡肌肉中脂肪酸代谢的节点基因,MAPK通路、ErbB通路和Herpes simplex infection通路可能是肌肉中脂肪酸代谢调控的关键通路,该结果为研究家禽肌肉中脂肪酸代谢的分子机制和进一步筛选优质肉质基因提供了基础资料。

Fatty acid metabolic products determine the flavor of chicken meat. Mining key genes regulating fatty acid metabolism has very important significance for improving meat quality and breeding new high quality broiler lines. The databases of KEGG and Wikipathway were used to analyze fatty acid metabolic networks, to find the key genes regulating fatty acid metabolism. The differential genes with co-expression of key genes in mieroarray in thigh muscle of chicken with different ages were filtered by Gene Spring（Pearson correlation coefficient over 0.9）, then imported to co-expression network by Cytoscape. Finally, the functions of key regulatory genes were found by across-species retrieval, the node genes and related pathways of fatty acid metabo- lism were mined in chicken muscle tissue. The results showed that Ep300, Traf2 and Pakl were node genes affecting fatty acid metabolism, and MAPK signaling pathway, ErbB signaling path- way and Herpes simplex infection pathway were critical pathways regulating fatty acid metabo- lism in chicken muscle tissue. These results provide basic information for studying the molecular mechanisms about fatty acid metabolism and for further screening quality meats genes in poultry.