新型凝集技术快速检测鸡白痢、鸡伤寒沙门菌

The New Agglutination Test for Rapid Detection of Salmonella pullorumand Salmonella gallinarum

为探求鸡白痢、鸡伤寒沙门菌快速检测的新型凝集技术，尝试用3种有机活性染料与抗体分别修饰二氧化硅活性微球制成免疫彩色二氧化硅微球（ICSN），构建快速检测鸡白痢、鸡伤寒沙门菌新型凝集技术。利用反相微乳法制备彩色二氧化硅纳米微球（CSN），用扫描电镜表征形貌特征和分散程度，通过凝集现象表征对鸡白痢、鸡伤寒沙门菌的检测效果。结果表明该新型凝集技术灵敏度好，其凝集结果醒目、直观、易于肉眼判别，且耗用抗体不到常规凝集试验的1／500，对目标菌的检测范围为10^2～10^9cfu·mL-1；重复性和稳定性好，4℃放置28d后，凝集效果与放置前无显著差异；具有较好的特异性和准确性。用ICSN构建的新型凝集技术具有灵敏、经济、稳定、简便、快速、准确等优点，不仅适用于快速检测鸡白痢、鸡伤寒沙门菌，还可为其它致病微生物快速检测提供基础模型。

The aim of this study was to develop a new agglutination test for the rapid detection of Salmonella pullorum and Salmonella gallinarum （ S. pullorum and S. gallinarum ）. In this study, three kinds of organic reactive dye and antibody were used to modify silica nanoparticles to prepare the immune colored silica nanoparticles （ICSN）, ICSN were used to build new agglutina- tion test for rapid detection of S. pullorum and S. gallinarum. The colored silica nanoparticles （CSN） were synthesized by reverse microemulsion method. The morphology and degree of dis- persion of CSN were characterized by SEM. The agglutination test was used to characterize the detection effect of S. pullorum and S. gallinarum. The results showed that the new agglutina- tion test was very sensitive, the aggregation results were prominently, intuitive and easy to dis- tinguish by naked eye, moreover, consumption of antibody in new agglutination test is only 1/500 of that consumed in traditional test, the linear range for S. pullorum and S. gallinarum was from 102 to 109cfu · mL 1. It was very stable and repeatable, after being stored at 4 ℃ for 28 days, the aggregation effect showed no significant difference; It also showed specificity and accu- racy. The new agglutination test was simple, fast, accurate, sensitive and economic. This new agglutination test could not only be used for detecting S. pullorum and S. gallinarum rapidly, but also provide a basis model for rapid detection of other pathogenic bacterium.