砷对兰州鲇组织中代谢酶活性及RNA和蛋白质含量影响

EFFECTS OF ARSENIC ON ACTIVITIES OF THE METABOLIC ENZYMES,CONTENTS OF RNA AND PROTEIN IN SEVERAL TISSUES OF SILURUS LANZHOUENSIS

采用毒性试验方法,研究了安全浓度(1.288 mg/L)条件下亚砷酸钠(NaAsO2)对兰州鲇(Silurus lanzhouensis)脑、鳃、肝脏、肌肉4种组织中6-磷酸葡萄糖脱氢酶(G-6-PDH)和乳酸脱氢酶(LDH)活性,以及RNA和蛋白质含量的影响。结果表明,染毒21d时,As(Ⅲ)可显著降低4种组织中G-6-PDH和LDH活性、RNA和蛋白质含量(P≤0.05)。撤毒后21d,除脑和肝组织中蛋白质含量未恢复到对照组水平(P≤0.05),肝脏中G-6-PDH活性超过了对照组水平(P≤0.05)外,其余各组织中G-6-PDH和LDH活性、RNA和蛋白质含量均可恢复到对照组水平(P>0.05)。以上结果表明,As(Ⅲ)对兰州鲇组织中代谢酶活性具有明显的抑制作用,可致组织细胞RNA损伤和可溶性蛋白质减少,但这种影响是可逆的,撤毒后一定时间内可恢复到正常水平。

The effects of Sodium arsenite on glucose-6-phosphate dehydrogenase （G-6-PDH） and lactate dehydrogenase （LDH） activities, RNA and protein contents in brain, gill, liver and muscle of Silurus lanzhouensis were investigated. The results indicated that during the 2 ld when the concentrations of Sodium arsenite were maintained safe in the water, G-6-PDH and LDH activities, RNA and protein contents decreased significantly in all tissues （P〈0.05）. Then, remaining fish were transferred to clean water for the elimination phase of 21 d. On the 21 st day after moving, compared with the control level, G-6-PDH and LDH activities, RNA and protein contents in brain, gill, liver and muscle were restored to the control level （P 〉 0.05）, except for the lower protein contents in brain and liver and higher G-6-PDH activity in liver （P〈 0.05）. These results suggested that arsenic could inhibit metabolic activity, induce RNA damage and the content of soluble protein, and this process was reversible after withdraw arsenic.